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Apoptosis‐associated speck‐like protein containing a caspase‐1 recruitment domain (ASC) contributes to osteoblast differentiation and osteogenesis

Sartoretto, Suelen ; Gemini‐Piperni, Sara ; da Silva, Rodrigo A. ; Calasans, Monica D. ; Rucci, Nadia ; Pires dos Santos, Thais M. ; Lima, Inayá B. C. ; Rossi, Alexandre M. ; Alves, Gutemberg ; Granjeiro, José M. ; Teti, Anna ; Zambuzzi, Willian F.

Journal of cellular physiology, 2019-04, Vol.234 (4), p.4140-4153 [Periódico revisado por pares]

United States: Wiley Subscription Services, Inc

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Título:
Apoptosis‐associated speck‐like protein containing a caspase‐1 recruitment domain (ASC) contributes to osteoblast differentiation and osteogenesis
Autor: Sartoretto, Suelen ; Gemini‐Piperni, Sara ; da Silva, Rodrigo A. ; Calasans, Monica D. ; Rucci, Nadia ; Pires dos Santos, Thais M. ; Lima, Inayá B. C. ; Rossi, Alexandre M. ; Alves, Gutemberg ; Granjeiro, José M. ; Teti, Anna ; Zambuzzi, Willian F.
Assuntos: 3T3 Cells ; Alkaline phosphatase ; Animals ; Apoptosis ; ASC ; Biocompatibility ; Biomarkers ; Biomedical materials ; bone formation ; Bone healing ; Bone morphogenetic protein 7 ; Bone Morphogenetic Protein 7 - pharmacology ; CARD Signaling Adaptor Proteins - deficiency ; CARD Signaling Adaptor Proteins - genetics ; CARD Signaling Adaptor Proteins - metabolism ; Caspase ; Cell Differentiation - drug effects ; Defects ; Differentiation ; Disease Models, Animal ; Female ; Fracture Healing ; Genotypes ; Healing ; inflammasome ; Inflammasomes ; Inflammasomes - drug effects ; Inflammasomes - metabolism ; Inflammation Mediators - metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; osteoblast ; Osteoblastogenesis ; Osteoblasts - drug effects ; Osteoblasts - metabolism ; Osteoblasts - pathology ; Osteogenesis ; Osteogenesis - drug effects ; Phenotypes ; Proteins ; Recruitment ; regenerative medicine ; Rodents ; Signal Transduction ; Tibia ; Tibia - metabolism ; Tibia - pathology ; Tibia - physiopathology ; Tibial Fractures - genetics ; Tibial Fractures - metabolism ; Tibial Fractures - pathology ; Tibial Fractures - physiopathology ; Time Factors
É parte de: Journal of cellular physiology, 2019-04, Vol.234 (4), p.4140-4153
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SourceType-Scholarly Journals-1
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Descrição: The role of apoptosis‐associated speck‐like protein containing a caspase‐1 recruitment domain (ASC) in bone healing remains to be understood. To address this issue, we investigated the requirement of inflammasome‐related genes in response to bone morphogenetic protein 7 (BMP7)‐induced osteoblast differentiation in vitro. To validate the importance of ASC on osteogenesis, we subjected wild‐type (WT) and ASC knockout C57BL/6 mice (ASC KO) to tibia defect to evaluate the bone healing process (up to 28 days). Our in vitro data showed that there is an involvement of ASC during BMP7‐induced osteoblast differentiation, concomitant to osteogenic biomarker expression. Indeed, primary osteogenic cells from ASC KO presented a lower osteogenic profile than those obtained from WT mice. To validate this hypothesis, we evaluated the bone healing process of tibia defects on both WT and ASC KO mice genotypes and the ASC KO mice were not able to fully heal tibia defects up to 28 days, whereas WT tibia defects presented a higher bone de novo volume at this stage, evidencing ASC as an important molecule during osteogenic phenotype. In addition, we have shown a higher involvement of runt‐related transcription factor 2 in WT sections during bone repair, as well as circulating bone alkaline phosphatase isoform when both were compared with ASC KO mice behavior. Altogether, our results showed for the first time the involvement of inflammasome during osteoblast differentiation and osteogenesis, which opens new avenues to understand the pathways involved in bone healing. ASC contributes to osteoblast differentiation and bone de novo deposition, opening new avenues to understand the complex mechanism coupling inflammation landscape and osteoblastogenesis during bone healing.
Editor: United States: Wiley Subscription Services, Inc
Idioma: Inglês

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Apoptosis‐associated speck‐like protein containing a caspase‐1 recruitment domain (ASC) contributes to osteoblast differentiation and osteogenesis

Sartoretto, Suelen ; Gemini‐Piperni, Sara ; da Silva, Rodrigo A. ; Calasans, Monica D. ; Rucci, Nadia ; Pires dos Santos, Thais M. ; Lima, Inayá B. C. ; Rossi, Alexandre M. ; Alves, Gutemberg ; Granjeiro, José M. ; Teti, Anna ; Zambuzzi, Willian F.

United States: Wiley Subscription Services, Inc

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