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Bacteriostatic Effect of Quercetin as an Antibiotic Alternative In Vivo and Its Antibacterial Mechanism In Vitro

Wang, Shengan ; Yao, Jiaying ; Zhou, Bo ; Yang, Jiaxin ; Chaudry, Maria T ; Wang, Mi ; Xiao, Fenglin ; Li, Yao ; Yin, Wenzhe

Journal of food protection, 2018-01, Vol.81 (1), p.68-78 [Periódico revisado por pares]

United States: Elsevier Limited

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Bacteriostatic Effect of Quercetin as an Antibiotic Alternative In Vivo and Its Antibacterial Mechanism In Vitro
Autor: Wang, Shengan ; Yao, Jiaying ; Zhou, Bo ; Yang, Jiaxin ; Chaudry, Maria T ; Wang, Mi ; Xiao, Fenglin ; Li, Yao ; Yin, Wenzhe
Assuntos: Alkaline phosphatase ; Animal production ; Animal welfare ; Animals ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Antiinfectives and antibacterials ; ATP ; Bacteria ; Bacteriostats ; Bifidobacterium - growth & development ; Biotechnology ; Cecum ; Cecum - microbiology ; Cell walls ; Chickens ; Chickens - microbiology ; Cloning ; Diet ; Diet - veterinary ; Drug resistance ; E coli ; Escherichia coli ; Escherichia coli - drug effects ; Feed additives ; Feeds ; Flavonoids ; Food ; Food safety ; Galactosidase ; Gram-negative bacteria ; Gram-Negative Bacteria - drug effects ; Gram-positive bacteria ; Lactobacillus ; Membranes ; Microbiota ; Microflora ; Microorganisms ; Minimum inhibitory concentration ; Mutation ; Poultry ; Pseudomonas aeruginosa ; Public health ; Quercetin ; Quercetin - pharmacology ; Random Allocation ; Salmonella ; Salmonella Typhimurium ; Salmonella typhimurium - growth & development ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Studies
É parte de: Journal of food protection, 2018-01, Vol.81 (1), p.68-78
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SourceType-Scholarly Journals-1
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Descrição: Quercetin, a ubiquitous flavonoid, is known to have antibacterial effects. The purpose of this study was to investigate the effect of quercetin on cecal microbiota of Arbor Acre (AA) broiler chickens in vivo and the bacteriostatic effect and antibacterial mechanism of quercetin in vitro. In vivo, 480 AA broilers (1 day old) were randomly allotted to four treatments (negative control and 0.2, 0.4, or 0.6 g of quercetin per kg of diet) for 42 days. Cecal microbial population and distribution were measured at the end of the experiment. The cecal microflora in these broilers included Proteobacteria, Fimicutes, Bacteroidetes, and Deferribacteres. Compared with the negative control, quercetin significantly decreased the copies of Pseudomonas aeruginosa ( P < 0.05), Salmonella enterica serotype Typhimurium ( P < 0.01), Staphylococcus aureus ( P < 0.01), and Escherichia coli ( P < 0.01) but significantly increased the copies of Lactobacillus ( P < 0.01), Bifidobacterium ( P < 0.01), and total bacteria ( P < 0.01). In vitro, we investigated the bacteriostatic effect of quercetin on four kinds of bacteria ( E. coli, P. aeruginosa, S. enterica Typhimurium, and S. aureus) and the antibacterial mechanism of quercetin in E. coli and S. aureus. The bacteriostatic effect of quercetin was stronger on gram-positive bacteria than on gram-negative bacteria. Quercetin damaged the cell walls and membranes of E. coli (at 50 × MIC) and S. aureus (at 10 × MIC). Compared with the control, the activity of the extracellular alkaline phosphatase and β-galactosidase and concentrations of soluble protein in E. coli and S. aureus were significantly increased (all P < 0.01), and the activity of ATP in S. aureus was significantly increased ( P < 0.01); however, no significant change in ATP activity in E. coli was observed ( P > 0.05). These results suggest that quercetin has potential as an alternative antibiotic feed additive in animal production.
Editor: United States: Elsevier Limited
Idioma: Inglês

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Bacteriostatic Effect of Quercetin as an Antibiotic Alternative In Vivo and Its Antibacterial Mechanism In Vitro

Wang, Shengan ; Yao, Jiaying ; Zhou, Bo ; Yang, Jiaxin ; Chaudry, Maria T ; Wang, Mi ; Xiao, Fenglin ; Li, Yao ; Yin, Wenzhe

United States: Elsevier Limited

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