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Toward dynamic structural biology: Two decades of single-molecule Förster resonance energy transfer

Lerner, Eitan ; Cordes, Thorben ; Ingargiola, Antonino ; Alhadid, Yazan ; Chung, SangYoon ; Michalet, Xavier ; Weiss, Shimon

Science (American Association for the Advancement of Science), 2018-01, Vol.359 (6373) [Periódico revisado por pares]

United States: The American Association for the Advancement of Science

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Toward dynamic structural biology: Two decades of single-molecule Förster resonance energy transfer
Autor: Lerner, Eitan ; Cordes, Thorben ; Ingargiola, Antonino ; Alhadid, Yazan ; Chung, SangYoon ; Michalet, Xavier ; Weiss, Shimon
Assuntos: Atomic force microscopy ; Atomic structure ; Biochemistry ; Biological activity ; Biological evolution ; Biological research ; Biology ; Biosensors ; Catalysis ; Chemical compounds ; Crystallography ; Deoxyribonucleic acid ; DNA ; DNA biosynthesis ; DNA repair ; DNA topoisomerase ; Drug screening ; Dynamic structural analysis ; Dynamics ; Electron microscopy ; Energy ; Energy transfer ; Enzymes ; Equilibrium ; Experiments ; Fluorescence ; Fluorescence resonance energy transfer ; Fluorescence Resonance Energy Transfer - history ; Fluorescence Resonance Energy Transfer - methods ; Fluorophores ; Folding ; High-throughput screening ; History, 20th Century ; History, 21st Century ; In vivo methods and tests ; Macromolecules ; Membrane proteins ; Microfluidics ; Microscopy ; Molecular biology ; Molecular Biology - trends ; Molecular motors ; NMR ; Nuclear magnetic resonance ; Nuclear reactions ; Nuclease ; Nucleic Acid Conformation ; Nucleic acids ; Protein Conformation ; Protein folding ; Proteins ; Repair & maintenance ; Replication ; Resonance ; Reviews ; Ribonucleic acid ; RNA ; Screening ; Single Molecule Imaging - history ; Single Molecule Imaging - methods ; Spectroscopy ; Subpopulations ; Transcription ; Translation
É parte de: Science (American Association for the Advancement of Science), 2018-01, Vol.359 (6373)
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
ObjectType-Review-3
content type line 23
Descrição: Classical structural biology can only provide static snapshots of biomacromolecules. Single-molecule Förster resonance energy transfer (smFRET) paved the way for studying dynamics in macromolecular structures under biologically relevant conditions. Since its first implementation in 1996, smFRET experiments have confirmed previously hypothesized mechanisms and provided new insights into many fundamental biological processes, such as DNA maintenance and repair, transcription, translation, and membrane transport. We review 22 years of contributions of smFRET to our understanding of basic mechanisms in biochemistry, molecular biology, and structural biology. Additionally, building on current state-of-the-art implementations of smFRET, we highlight possible future directions for smFRET in applications such as biosensing, high-throughput screening, and molecular diagnostics.
Editor: United States: The American Association for the Advancement of Science
Idioma: Inglês

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Toward dynamic structural biology: Two decades of single-molecule Förster resonance energy transfer

Lerner, Eitan ; Cordes, Thorben ; Ingargiola, Antonino ; Alhadid, Yazan ; Chung, SangYoon ; Michalet, Xavier ; Weiss, Shimon

United States: The American Association for the Advancement of Science

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