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Morinda citrifolia Linn. Reduces Parasite Load and Modulates Cytokines and Extracellular Matrix Proteins in C57BL/6 Mice Infected with Leishmania (Leishmania) amazonensis

Almeida-Souza, Fernando ; Cardoso, Flávia de Oliveira ; Souza, Bruno Vinicius da Conceição ; do Valle, Tânia Zaverucha ; de Sá, Joicy Cortez ; Oliveira, Iara Dos Santos da Silva ; de Souza, Celeste da Silva Freitas ; Moragas Tellis, Carla Junqueira ; Chagas, Maria do Socorro Dos Santos ; Behrens, Maria Dutra ; Abreu-Silva, Ana Lúcia ; Calabrese, Kátia da Silva Melby, Peter C.

PLoS neglected tropical diseases, 2016-08, Vol.10 (8), p.e0004900 [Periódico revisado por pares]

United States: Public Library of Science

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Morinda citrifolia Linn. Reduces Parasite Load and Modulates Cytokines and Extracellular Matrix Proteins in C57BL/6 Mice Infected with Leishmania (Leishmania) amazonensis
Autor: Almeida-Souza, Fernando ; Cardoso, Flávia de Oliveira ; Souza, Bruno Vinicius da Conceição ; do Valle, Tânia Zaverucha ; de Sá, Joicy Cortez ; Oliveira, Iara Dos Santos da Silva ; de Souza, Celeste da Silva Freitas ; Moragas Tellis, Carla Junqueira ; Chagas, Maria do Socorro Dos Santos ; Behrens, Maria Dutra ; Abreu-Silva, Ana Lúcia ; Calabrese, Kátia da Silva
Melby, Peter C.
Assuntos: Analysis ; Animals ; Antimicrobial agents ; Biological Products - administration & dosage ; Biological Products - adverse effects ; Biology and Life Sciences ; Care and treatment ; Collagen ; Cytokines ; Cytokines - blood ; Disease Models, Animal ; DNA, Protozoan - analysis ; Drugs ; Extracellular matrix ; Extracellular Matrix Proteins - analysis ; Female ; Fruit and Vegetable Juices - analysis ; Gas Chromatography-Mass Spectrometry ; Infections ; Leishmania - drug effects ; Leishmaniasis ; Leishmaniasis - drug therapy ; Liver - pathology ; Load ; Lymph Nodes - pathology ; Medicine and Health Sciences ; Mice ; Mice, Inbred C57BL ; Morinda - chemistry ; Parasite Load ; Parasites ; Parasitic diseases ; Proteins ; Risk factors ; Skin - pathology ; Studies ; Tropical diseases
É parte de: PLoS neglected tropical diseases, 2016-08, Vol.10 (8), p.e0004900
Notas: Conceived and designed the experiments: FAS MDB ALAS KdSC.Performed the experiments: FAS FdOC BVdCS JCdS IdSdSO CdSFdS CJMT MdSdSC.Analyzed the data: FAS TZdV.Contributed reagents/materials/analysis tools: MDB ALAS KdSC.Wrote the paper: FAS FdOC TZdV CJMT ALAS KdSC.
The authors have declared that no competing interests exist.
Descrição: The absence of an effective vaccine and the debilitating chemotherapy for Leishmaniasis demonstrate the need for developing alternative treatments. Several studies conducted with Morinda citrifolia have shown various biological activities, including antileishmanial activity, however its mechanisms of action are unknown. This study aimed to analyze the in vivo activity of M. citrifolia fruit juice (Noni) against Leishmania (Leishmania) amazonensis in C57BL/6 mice. M. citrifolia fruit juice from the Brazilian Amazon has shown the same constitution of other juices produced around the world and liquid chromatography-mass spectrometry analysis identified five compounds: deacetylasperulosidic acid, asperulosidic acid, rutin, nonioside B and nonioside C. Daily intragastric treatment with Noni was carried out after 55 days of L. (L.) amazonensis infection in C57BL/6 mice. Parasitic loads, cytokine and extracellular protein matrix expressions of the lesion site were analyzed by qPCR. Histopathology of the lesion site, lymph nodes and liver were performed to evaluate the inflammatory processes. Cytokines and biochemical parameters of toxicity from sera were also evaluated. The Noni treatment at 500 mg.kg-1.day-1 for 60 days decreased the lesion size and parasitic load in the footpad infected with L. (L.) amazonensis. The site of infection also showed decreased inflammatory infiltrates and decreased cytokine expressions for IL-12, TNF-α, TGF-β and IL-10. On the other hand, Noni treatment enhanced the extracellular matrix protein expressions of collagen IV, fibronectin and laminin in the infected footpad as well collagen I and II, fibronectin and laminin in the mock-infected footpads. No toxicity was observed at the end of treatment. These data show the efficacy of Noni treatment.
Editor: United States: Public Library of Science
Idioma: Inglês

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Morinda citrifolia Linn. Reduces Parasite Load and Modulates Cytokines and Extracellular Matrix Proteins in C57BL/6 Mice Infected with Leishmania (Leishmania) amazonensis

United States: Public Library of Science

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