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Complete genome sequence and analysis of Alcaligenes faecalis strain Mc250, a new potential plant bioinoculant

Felestrino, Érica Barbosa ; Sanchez, Angélica Bianchini ; Caneschi, Washington Luiz ; Lemes, Camila Gracyelle de Carvalho ; Assis, Renata de Almeida Barbosa ; Cordeiro, Isabella Ferreira ; Fonseca, Natasha Peixoto ; Villa, Morghana Marina ; Vieira, Izadora Tabuso ; Kamino, Luciana Hiromi Yoshino ; do Carmo, Flávio Fonseca ; da Silva, Aline Maria ; Thomas, Andrew Maltez ; Patané, José Salvatore Leister ; Ferreira, Fernanda Carla ; de Freitas, Leandro Grassi ; Varani, Alessandro de Mello ; Ferro, Jesus Aparecido ; Silva, Robson Soares ; Almeida, Nalvo Franco ; Garcia, Camila Carrião Machado ; Setubal, João Carlos ; Moreira, Leandro Marcio Ho, Paulo Lee

PloS one, 2020-11, Vol.15 (11), p.e0241546 [Peer Reviewed Journal]

United States: Public Library of Science

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Title:
Complete genome sequence and analysis of Alcaligenes faecalis strain Mc250, a new potential plant bioinoculant
Author: Felestrino, Érica Barbosa ; Sanchez, Angélica Bianchini ; Caneschi, Washington Luiz ; Lemes, Camila Gracyelle de Carvalho ; Assis, Renata de Almeida Barbosa ; Cordeiro, Isabella Ferreira ; Fonseca, Natasha Peixoto ; Villa, Morghana Marina ; Vieira, Izadora Tabuso ; Kamino, Luciana Hiromi Yoshino ; do Carmo, Flávio Fonseca ; da Silva, Aline Maria ; Thomas, Andrew Maltez ; Patané, José Salvatore Leister ; Ferreira, Fernanda Carla ; de Freitas, Leandro Grassi ; Varani, Alessandro de Mello ; Ferro, Jesus Aparecido ; Silva, Robson Soares ; Almeida, Nalvo Franco ; Garcia, Camila Carrião Machado ; Setubal, João Carlos ; Moreira, Leandro Marcio
Ho, Paulo Lee
Subjects: Agricultural bacteriology ; Alcaligenes ; Alcaligenes faecalis ; Alcaligenes faecalis - drug effects ; Alcaligenes faecalis - genetics ; Animals ; Anti-Bacterial Agents - pharmacology ; Bacteria ; Bacteriology, Agricultural ; Base Sequence ; Biodegradation ; Biological control ; Biology and Life Sciences ; Biosynthesis ; Burkholderia ; Chromosomes ; Citrus - microbiology ; Citrus - parasitology ; Citrus sinensis ; Denitrification ; Deoxyribonucleic acid ; DNA ; DNA repair ; DNA, Circular - genetics ; Drug Resistance, Microbial - drug effects ; Drug Resistance, Microbial - genetics ; Ectoine ; Eggs ; Emulsan ; Emulsans ; Endemic plants ; Environmental conditions ; Gene clusters ; Genes ; Genetic aspects ; Genome, Bacterial ; Genomes ; Genomic Islands - genetics ; Grasslands ; Hatching ; Health aspects ; Internalization ; Iron - metabolism ; Metabolism ; Metals, Heavy - toxicity ; Mimosa - microbiology ; Mimosa calodendron ; Nematoda - physiology ; Nematodes ; Nucleotide sequence ; Oxidative metabolism ; Oxidative stress ; Pathogens ; Phenolic compounds ; Phenols ; Phenols - metabolism ; Phenotypes ; Phylogeny ; Physical Sciences ; Physiological aspects ; Plant growth ; Proteins ; Research and Analysis Methods ; Resorcinol ; Siderophores ; Virulence ; Whole Genome Sequencing ; Xanthomonas citri
Is Part Of: PloS one, 2020-11, Vol.15 (11), p.e0241546
Notes: Competing Interests: The authors have declared that no competing interests exist.
Description: Here we present and analyze the complete genome of Alcaligenes faecalis strain Mc250 (Mc250), a bacterium isolated from the roots of Mimosa calodendron, an endemic plant growing in ferruginous rupestrian grasslands in Minas Gerais State, Brazil. The genome has 4,159,911 bp and 3,719 predicted protein-coding genes, in a single chromosome. Comparison of the Mc250 genome with 36 other Alcaligenes faecalis genomes revealed that there is considerable gene content variation among these strains, with the core genome representing only 39% of the protein-coding gene repertoire of Mc250. Mc250 encodes a complete denitrification pathway, a network of pathways associated with phenolic compounds degradation, and genes associated with HCN and siderophores synthesis; we also found a repertoire of genes associated with metal internalization and metabolism, sulfate/sulfonate and cysteine metabolism, oxidative stress and DNA repair. These findings reveal the genomic basis for the adaptation of this bacterium to the harsh environmental conditions from where it was isolated. Gene clusters associated with ectoine, terpene, resorcinol, and emulsan biosynthesis that can confer some competitive advantage were also found. Experimental results showed that Mc250 was able to reduce (~60%) the virulence phenotype of the plant pathogen Xanthomonas citri subsp. citri when co-inoculated in Citrus sinensis, and was able to eradicate 98% of juveniles and stabilize the hatching rate of eggs to 4% in two species of agricultural nematodes. These results reveal biotechnological potential for the Mc250 strain and warrant its further investigation as a biocontrol and plant growth-promoting bacterium.
Publisher: United States: Public Library of Science
Language: English

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Complete genome sequence and analysis of Alcaligenes faecalis strain Mc250, a new potential plant bioinoculant

United States: Public Library of Science

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