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Co‐initiators of polymerization can modulate the inflammatory cytokine release without major cytotoxic effects in human dental pulp cells

Agostinelli, Bárbara Gabriela ; Andia, Denise Carleto ; Lima, Adriano F.

Journal of biomedical materials research. Part B, Applied biomaterials, 2023-05, Vol.111 (5), p.1112-1120 [Periódico revisado por pares]

Hoboken, USA: John Wiley & Sons, Inc

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  • Título:
    Co‐initiators of polymerization can modulate the inflammatory cytokine release without major cytotoxic effects in human dental pulp cells
  • Autor: Agostinelli, Bárbara Gabriela ; Andia, Denise Carleto ; Lima, Adriano F.
  • Assuntos: Antineoplastic Agents ; Apoptosis ; Benzoates ; Benzoic acid ; Biomedical materials ; Carbon dioxide ; Cell culture ; Cell death ; Cells, Cultured ; Cytokines ; Cytotoxicity ; Dental Pulp ; DMAEMA ; EDAB ; Exposure ; Flow cytometry ; Humans ; Inflammation ; Inflammatory response ; Initiators ; Interleukin 6 ; Interleukin-8 ; iodonium ; Materials research ; Materials science ; mediators ; Metabolism ; Mitochondria ; Molars ; Mortality ; Polymerization ; Toxicity ; Variance analysis
  • É parte de: Journal of biomedical materials research. Part B, Applied biomaterials, 2023-05, Vol.111 (5), p.1112-1120
  • Notas: Funding information
    Fundação de Amparo à Pesquisa do Estado de São Paulo, Grant/Award Number: 2020/14665‐8
  • Descrição: To evaluate the cytotoxicity of co‐initiators of polymerization and its influence on cytokine release from human dental pulp cells (hDPCs). Cells were isolated from the dental pulp of sound human third molars. The co‐initiators dimethylaminoethyl amine benzoate—(EDAB), 2‐(dimethylamino)ethyl methacrylate (DMAEMA); 2‐Ethylhexyl 4‐(dimethylamino)benzoate (EHA) and bis(4‐methyl phenyl)iodonium hexafluorophosphate (BPI) were diluted in dimethylsulfoxide (DMSO) at different concentrations. In this way, experimental groups and one control (without treatment) were obtained. hDPCs (10 × 104 cell per well) were seeded on 96 well plates and incubated at 37°C and 5% CO2 for 48 h. After this, the cells were exposed to different concentrations of co‐initiators cited for 24 h. After this time, the culture medium was removed, and the mitochondrial metabolism was evaluated by MTT assay, cell death by flow cytometry, and cytokine released (IL‐1β, IL6, IL‐8, IL‐10, and TNF‐α) was analyzed by MAGPIX assay. The data were analyzed by ANOVA one‐way and Tukey's test. EHA, DMAEMA, and EDAB did not reduce the mitochondrial metabolism. BPI presented high toxicity with remarkable reduction (80%) after exposure to 1 mM. The cell death of all test groups was similar to control. After 24 h treatment, the IL‐8 was up‐regulated by all compounds, while IL‐6 was upregulated after exposure to EHA and downregulated after DMAEMA stimulation. BPI, EHA, EDAB, and DMAEMA can trigger an initial inflammatory response, upregulating the IL‐8 secretion in hDPCs in a compound‐concentration‐dependent manner; however, this was not accompanied by major cytotoxic effects at cell death or mitochondrial‐metabolism levels.
  • Editor: Hoboken, USA: John Wiley & Sons, Inc
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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