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Enhanced genetic tools for engineering multigene traits into green algae

Rasala, Beth A ; Chao, Syh-Shiuan ; Pier, Matthew ; Barrera, Daniel J ; Mayfield, Stephen P Chen, Wei Ning

PloS one, 2014-04, Vol.9 (4), p.e94028-e94028 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Enhanced genetic tools for engineering multigene traits into green algae
Autor: Rasala, Beth A ; Chao, Syh-Shiuan ; Pier, Matthew ; Barrera, Daniel J ; Mayfield, Stephen P
Chen, Wei Ning
Assuntos: Algae ; Amino acids ; Analysis ; Animal nutrition ; Aquatic microorganisms ; BASIC BIOLOGICAL SCIENCES ; Biodiesel fuels ; Biology and Life Sciences ; Biosynthesis ; Biotechnology ; Biotechnology - methods ; Chlamydomonas ; Chlamydomonas reinhardtii ; Chlamydomonas reinhardtii - genetics ; Chloroplasts ; Cloning ; Commercialization ; Drugs ; Endoplasmic reticulum ; Engineering and Technology ; Expression vectors ; Gene expression ; Genetic engineering ; Genetic Engineering - methods ; Genetic Enhancement ; Genetic transformation ; Genetic vectors ; Genetically modified organisms ; Genomes ; Genomics ; Localization ; Metabolic engineering ; Metabolism ; Microscopy ; Mitochondria ; Nutrition ; Peptides ; Proteins ; Respiration ; Science & Technology - Other Topics ; Transformation ; Transgenes ; Transgenic ; Transgenic engineering
É parte de: PloS one, 2014-04, Vol.9 (4), p.e94028-e94028
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
California Energy Commission
EE0003373; CILMSF #500-10-039
Conceived and designed the experiments: BAR SPM. Performed the experiments: BAR SC MP DJB. Analyzed the data: BAR SC MP DJB SPM. Wrote the paper: BAR.
Competing Interests: The authors have declared that no competing interests exist.
Descrição: Transgenic microalgae have the potential to impact many diverse biotechnological industries including energy, human and animal nutrition, pharmaceuticals, health and beauty, and specialty chemicals. However, major obstacles to sophisticated genetic and metabolic engineering in algae have been the lack of well-characterized transformation vectors to direct engineered gene products to specific subcellular locations, and the inability to robustly express multiple nuclear-encoded transgenes within a single cell. Here we validate a set of genetic tools that enable protein targeting to distinct subcellular locations, and present two complementary methods for multigene engineering in the eukaryotic green microalga Chlamydomonas reinhardtii. The tools described here will enable advanced metabolic and genetic engineering to promote microalgae biotechnology and product commercialization.
Editor: United States: Public Library of Science
Idioma: Inglês

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Enhanced genetic tools for engineering multigene traits into green algae

Rasala, Beth A ; Chao, Syh-Shiuan ; Pier, Matthew ; Barrera, Daniel J ; Mayfield, Stephen P Chen, Wei Ning

United States: Public Library of Science

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