Idioma:

Tandem Mass Spectrometry–Based Amyloid Typing Using Manual Microdissection and Open-Source Data Processing

Phipps, William S ; Smith, Kelly D ; Yang, Han-Yin ; Henderson, Clark M ; Pflaum, Hannah ; Lerch, Melissa L ; Fondrie, William E ; Emrick, Michelle A ; Wu, Christine C ; MacCoss, Michael J ; Noble, William S ; Hoofnagle, Andrew N

American journal of clinical pathology, 2022-05, Vol.157 (5), p.748-757 [Periódico revisado por pares]

US: Oxford University Press

Texto completo disponível

Citações Citado por

Enviar para

Título:
Tandem Mass Spectrometry–Based Amyloid Typing Using Manual Microdissection and Open-Source Data Processing
Autor: Phipps, William S ; Smith, Kelly D ; Yang, Han-Yin ; Henderson, Clark M ; Pflaum, Hannah ; Lerch, Melissa L ; Fondrie, William E ; Emrick, Michelle A ; Wu, Christine C ; MacCoss, Michael J ; Noble, William S ; Hoofnagle, Andrew N
Assuntos: Amyloid - analysis ; Amyloidogenesis ; Amyloidogenic Proteins ; Amyloidosis ; Amyloidosis - diagnosis ; Data processing ; Electronic data processing ; Handbooks, vade-mecums, etc ; Humans ; Liquid chromatography ; Mass spectrometry ; Mass spectroscopy ; Medical laboratories ; Microdissection ; Original ; Proteins ; Proteomics ; Sampling ; Scientific imaging ; Spectrum analysis ; Tandem Mass Spectrometry - methods ; Typing
É parte de: American journal of clinical pathology, 2022-05, Vol.157 (5), p.748-757
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Currently at Discovery Attribute Sciences, Therapeutic Discovery, Amgen, South San Francisco, CA, USA.
Descrição: Abstract Objectives Standard implementations of amyloid typing by liquid chromatography–tandem mass spectrometry use capabilities unavailable to most clinical laboratories. To improve accessibility of this testing, we explored easier approaches to tissue sampling and data processing. Methods We validated a typing method using manual sampling in place of laser microdissection, pairing the technique with a semiquantitative measure of sampling adequacy. In addition, we created an open-source data processing workflow (Crux Pipeline) for clinical users. Results Cases of amyloidosis spanning the major types were distinguishable with 100% specificity using measurements of individual amyloidogenic proteins or in combination with the ratio of λ and κ constant regions. Crux Pipeline allowed for rapid, batched data processing, integrating the steps of peptide identification, statistical confidence estimation, and label-free protein quantification. Conclusions Accurate mass spectrometry–based amyloid typing is possible without laser microdissection. To facilitate entry into solid tissue proteomics, newcomers can leverage manual sampling approaches in combination with Crux Pipeline and related tools.
Editor: US: Oxford University Press
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Anterior Resultado 7 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Tandem Mass Spectrometry–Based Amyloid Typing Using Manual Microdissection and Open-Source Data Processing

Phipps, William S ; Smith, Kelly D ; Yang, Han-Yin ; Henderson, Clark M ; Pflaum, Hannah ; Lerch, Melissa L ; Fondrie, William E ; Emrick, Michelle A ; Wu, Christine C ; MacCoss, Michael J ; Noble, William S ; Hoofnagle, Andrew N

US: Oxford University Press

Buscando em bases de dados remotas. Favor aguardar.