Language:

Anti–Trypanosoma cruzi effect of the photodynamic antiparasitic chemotherapy using phenothiazine derivatives as photosensitizers

Barbosa, Artur F. S. ; Santos, Ivanilson P. ; Santos, Gustavo M. P. ; Bastos, Tanira M. ; Rocha, Vinícius. P. C. ; Meira, Cássio S. ; Soares, Milena B. P. ; Pitta, Ivan R. ; Pinheiro, Antônio Luiz Barbosa

Lasers in medical science, 2020-02, Vol.35 (1), p.79-85 [Peer Reviewed Journal]

London: Springer London

Full text available

Citations Cited by

Actions
1. Add to e-Shelf
2. Remove from e-Shelf
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
Anti–Trypanosoma cruzi effect of the photodynamic antiparasitic chemotherapy using phenothiazine derivatives as photosensitizers
Author: Barbosa, Artur F. S. ; Santos, Ivanilson P. ; Santos, Gustavo M. P. ; Bastos, Tanira M. ; Rocha, Vinícius. P. C. ; Meira, Cássio S. ; Soares, Milena B. P. ; Pitta, Ivan R. ; Pinheiro, Antônio Luiz Barbosa
Subjects: Animals ; Anti-Infective Agents - pharmacology ; Antiparasitic Agents - pharmacology ; Antiparasitic Agents - therapeutic use ; Benznidazole ; Cell Death - drug effects ; Cell Death - radiation effects ; Chagas Disease - drug therapy ; Chagas' disease ; Chemotherapy ; Cytotoxicity ; Dentistry ; Derivatives ; Flux density ; Humans ; Image analysis ; Image processing ; Lasers ; Light ; Macrophages ; Medicine ; Medicine & Public Health ; Methylene blue ; Methylene Blue - chemistry ; Methylene Blue - pharmacology ; Methylene Blue - therapeutic use ; Mice, Inbred BALB C ; Optical Devices ; Optics ; Original Article ; Parasite Load ; Parasitic diseases ; Phenothiazine ; Phenothiazines - pharmacology ; Phenothiazines - therapeutic use ; Photoactivation ; Photochemotherapy ; Photonics ; Photosensitizing Agents - pharmacology ; Photosensitizing Agents - therapeutic use ; Quantum Optics ; Side effects ; Tolonium Chloride - chemistry ; Tolonium Chloride - pharmacology ; Tolonium Chloride - therapeutic use ; Toluidine ; Toluidine blue ; Toxicity ; Trypanosoma cruzi - drug effects ; Trypanosoma cruzi - radiation effects ; Vector-borne diseases
Is Part Of: Lasers in medical science, 2020-02, Vol.35 (1), p.79-85
Notes: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Description: Chagas disease is endemic in Latin America and increasingly found in non-endemic countries. Its treatment is limited due to the variable efficacy and several side effects of benznidazole. Photodynamic antimicrobial chemotherapy (PACT) may be an attractive approach for treating Chagas disease. Here, the trypanocidal activity of PACT was investigated in vitro using phenothiazine derivatives. The cytotoxicity of both, methylene blue (MB) and toluidine blue (TBO), was determined on macrophages cultures using AlamarBlue method. The trypanocidal activity of the two photosensitizers was initially evaluated by determining their IC 50 values against trypomastigote forms. After this, the trypanocidal effect was evaluated in cultures of infected macrophages using an automatized image analysis protocol. All experiments were performed in the dark and in the clear phase (after a photodynamic exposure). The compounds showed no cytotoxicity in both phases at the tested concentrations. The IC 50 values for the sole use of MB and TBO were 2.6 and 1.2 μM, respectively. The photoactivation of the compounds using a fixed energy density (J/cm 2 ) caused a reduction of the IC 50 values to 1.0 and 0.9 μM, respectively. It was found that, on infected macrophage, the use of TBO significantly reduced the number of infected cells and parasitic load, and this effect was increased in the presence of light. The results of the present study are indicative that PACT may be considered as both selective and effective therapeutic intervention for treating Chagas disease.
Publisher: London: Springer London
Language: English

Links

View this record in MEDLINE/PubMed

Back to results list

Previous Result 8 Next Go to Next page

Implemented by: Social Network Logos:

Anti–Trypanosoma cruzi effect of the photodynamic antiparasitic chemotherapy using phenothiazine derivatives as photosensitizers

Barbosa, Artur F. S. ; Santos, Ivanilson P. ; Santos, Gustavo M. P. ; Bastos, Tanira M. ; Rocha, Vinícius. P. C. ; Meira, Cássio S. ; Soares, Milena B. P. ; Pitta, Ivan R. ; Pinheiro, Antônio Luiz Barbosa

London: Springer London

Searching Remote Databases, Please Wait