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Capacity of the medullary cavity of tibia and femur for intra-bone marrow transplantation in mice

Fink, Dieter ; Pfeiffenberger, Ulrike ; Bernthaler, Tina ; Schober, Sophie ; Thonhauser, Kerstin E ; Rülicke, Thomas Bunting, Kevin D

PloS one, 2019-11, Vol.14 (11), p.e0224576-e0224576 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Capacity of the medullary cavity of tibia and femur for intra-bone marrow transplantation in mice
Autor: Fink, Dieter ; Pfeiffenberger, Ulrike ; Bernthaler, Tina ; Schober, Sophie ; Thonhauser, Kerstin E ; Rülicke, Thomas
Bunting, Kevin D
Assuntos: Animal models ; Animal sciences ; Animals ; Biology and Life Sciences ; Bioluminescence ; Blood circulation ; Blood vessels ; Boltzmann, Ludwig Eduard (1844-1906) ; Bone marrow ; Bone marrow transplantation ; Bone Marrow Transplantation - methods ; Cell Movement - physiology ; Cells (biology) ; EDTA ; Effective capacity ; Emission ; Emission measurements ; Emissions ; Emissions (Pollution) ; Ethylenediaminetetraacetic acid ; Femur ; Femur - physiopathology ; Femur - transplantation ; Hematopoietic stem cell transplantation ; Hematopoietic Stem Cell Transplantation - methods ; Hematopoietic stem cells ; Hematopoietic Stem Cells - cytology ; Hematopoietic Stem Cells - metabolism ; Humans ; Injection ; Laboratory animals ; Leukemia ; Luciferase ; Medicine and Health Sciences ; Mice ; Physical Sciences ; Progenitor cells ; Research and Analysis Methods ; Stem cell transplantation ; Stem cells ; Tibia ; Tibia - physiopathology ; Tibia - transplantation ; Transplantation ; Transplantation, Heterologous - methods ; Transplants & implants ; Veterinary medicine ; Zoology
É parte de: PloS one, 2019-11, Vol.14 (11), p.e0224576-e0224576
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: The authors have declared that no competing interests exist.
Descrição: Intra-bone marrow transplantation (IBMT) has been adapted for mouse models to improve the seeding efficiency of transplanted hematopoietic stem and progenitor cells. Commonly used injection volumes for IBMT into the tibia differ between 10 and 40 μL even though considerable amounts of injected cells leak into the blood circulation immediately after injection. Injection of 3 μL trypan blue into the tibia of dead BALB/c mice showed staining in large vessels of hind limbs, even without supporting circulation. We therefore tested the effective capacity of the medullary cavity of dissected tibiae and femora of different mouse strains by bioluminescence imaging after injection of luciferase expressing cells. Cell leakage was already observed at 3 μL of injection volume and the measured emission rate increased significantly when 5 and 10 μL of volume with the same cell concentration were injected. Surprisingly, increasing injection volumes containing constant cell amounts resulted in comparable emission rates, suggesting a similar amount of leaked and absorbed cells independent of the injection volume. However, the absorption of a specific amount of injected cells could not be confirmed, as the ratio of leaked to absorbed cells was similar between IBMT that were performed with a constant injection volume containing either low or high cell amounts. In summary, for optimal cell transplantation via IBMT in mice we suggest to inject a high concentrated cell suspension with a maximum injection volume of 3 μL.
Editor: United States: Public Library of Science
Idioma: Inglês

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Capacity of the medullary cavity of tibia and femur for intra-bone marrow transplantation in mice

Fink, Dieter ; Pfeiffenberger, Ulrike ; Bernthaler, Tina ; Schober, Sophie ; Thonhauser, Kerstin E ; Rülicke, Thomas Bunting, Kevin D

United States: Public Library of Science

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