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A Comparison of Microbial Water Quality and Diversity for Ballast and Tropical Harbor Waters

Ng, Charmaine ; Le, Thai-Hoang ; Goh, Shin Giek ; Liang, Liang ; Kim, Yiseul ; Rose, Joan B ; Yew-Hoong, Karina Gin Almeida, Adelaide

PloS one, 2015-11, Vol.10 (11), p.e0143123-e0143123 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
A Comparison of Microbial Water Quality and Diversity for Ballast and Tropical Harbor Waters
Autor: Ng, Charmaine ; Le, Thai-Hoang ; Goh, Shin Giek ; Liang, Liang ; Kim, Yiseul ; Rose, Joan B ; Yew-Hoong, Karina Gin
Almeida, Adelaide
Assuntos: Anti-Bacterial Agents - pharmacology ; Antibiotic resistance ; Antibiotics ; Antimicrobial agents ; Aquaculture ; Bacteria ; Bacteria - drug effects ; Bacteria - genetics ; Bacterial Proteins - genetics ; Ballast ; Ballast tanks ; Biodiversity ; Chloramphenicol ; Chloramphenicol - pharmacology ; Cluster Analysis ; Coastal environments ; Cyanobacteria ; Drug resistance ; Drug Resistance, Microbial ; E coli ; Engineering ; Enterococcus - drug effects ; Enterococcus - genetics ; Environmental engineering ; Escherichia coli ; Escherichia coli - drug effects ; Escherichia coli - genetics ; Farms ; Florfenicol ; Genes ; Harbors ; High-Throughput Nucleotide Sequencing ; Hydrocarbon-degrading bacteria ; Indicators ; Microbiology ; Microorganisms ; Nonnative species ; Oceans ; Organisms ; Principal Component Analysis ; Pseudomonas aeruginosa ; Quality assessment ; Real-Time Polymerase Chain Reaction ; Retention tanks ; Retention time ; RNA, Ribosomal, 16S - analysis ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Salmonella ; Salmonella - genetics ; Seawater - microbiology ; Sediments ; Sequence Analysis, DNA ; Ships ; Sulfonamides ; Sulfonamides - pharmacology ; Surface water ; Trimethoprim ; Trimethoprim - pharmacology ; Viability ; Vibrio ; Vibrio - genetics ; Water analysis ; Water Quality ; Water quality assessments ; Water sampling ; Water tanks ; Water-borne diseases ; Waterborne diseases
É parte de: PloS one, 2015-11, Vol.10 (11), p.e0143123-e0143123
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: The authors have declared that no competing interests exist.
Conceived and designed the experiments: CN THL SGG LL YK JBR KGYH. Performed the experiments: CN THL SGG LL YK. Analyzed the data: CN THL SGG LL. Contributed reagents/materials/analysis tools: JBR KGYH. Wrote the paper: CN THL JBR KGYH.
Descrição: Indicator organisms and antibiotic resistance were used as a proxy to measure microbial water quality of ballast tanks of ships, and surface waters in a tropical harbor. The survival of marine bacteria in ballast tanks appeared to diminish over longer water retention time, with a reduction of cell viability observed after a week based on heterotrophic plate counts. Pyrosequencing of 16S rRNA genes showed distinct differences in microbial composition of ballast and harbor waters. The harbor waters had a higher abundance of operational taxonomic units (OTUs) assigned to Cyanobacteria (Synechococcus spp.) and α-proteobacteria (SAR11 members), while marine hydrocarbon degraders such as γ-proteobacteria (Ocenspirillaes spp., Thiotrchales spp.) and Bacteroidetes (Flavobacteriales spp.) dominated the ballast water samples. Screening of indicator organisms found Escherichia coli (E. coli), Enterococcus and Pseudomonas aeruginosa (P. aeruginosa) in two or more of the ballast and harbor water samples tested. Vibrio spp. and Salmonella spp. were detected exclusively in harbor water samples. Using quantitative PCR (qPCR), we screened for 13 antibiotic resistant gene (ARG) targets and found higher abundances of sul1 (4.13-3.44 x 102 copies/mL), dfrA (0.77-1.80 x10 copies/mL) and cfr (2.00-5.21 copies/mL) genes compared to the other ARG targets selected for this survey. These genes encode for resistance to sulfonamides, trimethoprim and chloramphenicol-florfenicol antibiotics, which are also known to persist in sediments of aquaculture farms and coastal environments. Among the ARGs screened, we found significant correlations (P<0.05) between ereA, ermG, cfr and tetO genes to one or more of the indicator organisms detected in this study, which may suggest that these members contribute to the environmental resistome. This study provides a baseline water quality survey, quantitatively assessing indicators of antibiotic resistance, potentially pathogenic organisms and a broad-brush description of difference in microbial composition and diversity between open oceans and tropical coastal environments through the use of next generation sequencing technology.
Editor: United States: Public Library of Science
Idioma: Inglês

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A Comparison of Microbial Water Quality and Diversity for Ballast and Tropical Harbor Waters

Ng, Charmaine ; Le, Thai-Hoang ; Goh, Shin Giek ; Liang, Liang ; Kim, Yiseul ; Rose, Joan B ; Yew-Hoong, Karina Gin Almeida, Adelaide

United States: Public Library of Science

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