Idioma:

Oligonucleotide and Polymer Functionalized Nanoparticles for Amplification-Free Detection of DNA

Thomson, David A. C ; Tee, Ernest H. L ; Tran, Nguyen T. D ; Monteiro, Michael J ; Cooper, Matthew A

Biomacromolecules, 2012-06, Vol.13 (6), p.1981-1989 [Periódico revisado por pares]

Washington, DC: American Chemical Society

Texto completo disponível

Citações Citado por

Enviar para

Título:
Oligonucleotide and Polymer Functionalized Nanoparticles for Amplification-Free Detection of DNA
Autor: Thomson, David A. C ; Tee, Ernest H. L ; Tran, Nguyen T. D ; Monteiro, Michael J ; Cooper, Matthew A
Assuntos: Applied sciences ; Biological and medical sciences ; Biosensors ; Biotechnology ; Click Chemistry ; DNA Probes - chemistry ; DNA, Single-Stranded - analysis ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Herpes simplex virus ; Methods. Procedures. Technologies ; Nanoparticles - chemistry ; Oligonucleotides - chemistry ; Organic polymers ; Physicochemistry of polymers ; Polymers - chemical synthesis ; Polymers - chemistry ; Polymers with particular properties ; Preparation, kinetics, thermodynamics, mechanism and catalysts ; Simplexvirus - chemistry ; Various methods and equipments
É parte de: Biomacromolecules, 2012-06, Vol.13 (6), p.1981-1989
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Article-2
ObjectType-Feature-1
Descrição: Sensitive and quantitative nucleic acid testing from complex biological samples is now an important component of clinical diagnostics. Whereas nucleic acid amplification represents the gold standard, its utility in resource-limited and point-of-care settings can be problematic due to assay interferants, assay time, engineering constraints, and costs associated with both wetware and hardware. In contrast, amplification-free nucleic acid testing can circumvent these limitations by enabling direct target hybridization within complex sample matrices. In this work, we grew random copolymer brushes from the surface of silica-coated magnetic nanoparticles using azide-modified and hydroxyl oligo ethylene glycol methacrylate (OEGMA) monomers. The azide-functionalized polymer brush was first conjugated, via copper-catalyzed azide/alkyne cycloaddition (CuAAC), with herpes simplex virus (HSV)-specific oligonucleotides and then with alkyne-substituted polyethylene glycol to eliminate all residual azide groups. Our methodology enabled control over brush thickness and probe density and enabled multiple consecutive coupling reactions on the particle grafted brush. Brush- and probe-modified particles were then combined in a 20 min hybridization with fluorescent polystyrene nanoparticles modified with HSV-specific reporter probes. Following magnetic capture and washing, the particles were analyzed with an aggregate fluorescence measurement, which yielded a limit of detection of 6 pM in buffer and 60 pM in 50% fetal bovine serum. Adoption of brush- and probe-modified particles into a particle counting assay will result in the development of diagnostic assays with significant improvements in sensitivity.
Editor: Washington, DC: American Chemical Society
Idioma: Inglês

Links

Voltar para lista de resultados

Anterior Resultado 9 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Oligonucleotide and Polymer Functionalized Nanoparticles for Amplification-Free Detection of DNA

Thomson, David A. C ; Tee, Ernest H. L ; Tran, Nguyen T. D ; Monteiro, Michael J ; Cooper, Matthew A

Washington, DC: American Chemical Society

Buscando em bases de dados remotas. Favor aguardar.