Idioma:

Denaturation and renaturation of a β-1,6:1,3-glucan, lentinan, associated with expression of T-cell-mediated responses

MAEDA, Y. Y ; WATANABE, S. T ; CHIHARA, C ; ROKUTANDA, M

Cancer research (Chicago, Ill.), 1988-02, Vol.48 (3), p.671-675 [Periódico revisado por pares]

Philadelphia, PA: American Association for Cancer Research

Texto completo disponível

Citações Citado por

Enviar para

Título:
Denaturation and renaturation of a β-1,6:1,3-glucan, lentinan, associated with expression of T-cell-mediated responses
Autor: MAEDA, Y. Y ; WATANABE, S. T ; CHIHARA, C ; ROKUTANDA, M
Assuntos: Acute-Phase Proteins - biosynthesis ; Animals ; Antineoplastic agents ; Biological and medical sciences ; Dimethyl Sulfoxide - pharmacology ; Female ; General aspects ; Hemorrhage - chemically induced ; Immunity, Cellular ; Immunotherapy ; Lentinan - immunology ; Lentinan - therapeutic use ; Medical sciences ; Mice ; Mice, Inbred Strains ; Molecular Conformation ; Neoplasms, Experimental - therapy ; Pharmacology. Drug treatments ; Polysaccharides - immunology ; Sodium Hydroxide - pharmacology ; Structure-Activity Relationship ; T-Lymphocytes - immunology ; Urea - pharmacology
É parte de: Cancer research (Chicago, Ill.), 1988-02, Vol.48 (3), p.671-675
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
Descrição: Correlation between the higher structure and biological functions of lentinan, a beta-1,6;1,3-glucan capable of potentiating T- and non-T-cell-mediated responses, were investigated by measurements of optical rotation and some biological responses. The addition of urea or dimethyl sulfoxide decreased specific rotation at 589 nm, [alpha]D, of lentinan in a concentration-dependent manner and the removal of these denaturants resulted in the recovery of [alpha]D values. Measurements of optical rotatory dispersion in the spectral region between 600 and 200 nm showed the change in the higher structure of lentinan more clearly. Denaturation and renaturation of lentinan using urea and dimethyl sulfoxide were associated with the decrease and the recovery of antitumor activity against P-815 mastocytoma and vascular dilation and hemorrhage-inducing activity, found to be T-cell-mediated responses. Lentinan was also denatured by NaOH and the transition of [alpha]D values and optical rotatory dispersion curves were seen in the manner of two concentration-dependent phases. Removal of NaOH led to the recovery of optical rotation of lentinan and its antitumor and vascular dilation and hemorrhage-inducing activity. However, recovery of these bioactivities was more difficult in the case of the higher concentrations of NaOH above 2% than the lower ones. During the process of renaturation of lentinan, random aggregation may take place. An increase of serum acute phase proteins, a non-T-cell-mediated response caused by lentinan, was not affected by the change of the higher structure of lentinan.
Editor: Philadelphia, PA: American Association for Cancer Research
Idioma: Inglês

Links

Voltar para lista de resultados

Resultado 1 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Denaturation and renaturation of a β-1,6:1,3-glucan, lentinan, associated with expression of T-cell-mediated responses

MAEDA, Y. Y ; WATANABE, S. T ; CHIHARA, C ; ROKUTANDA, M

Philadelphia, PA: American Association for Cancer Research

Buscando em bases de dados remotas. Favor aguardar.