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Production of alkaline and acidic pectinases by Saccharomyces cerevisiae

K. F. Oliveira C. S Souza; Elisabete José Vicente C. Laluce; Sociedade Brasileira de Bioquímica e Biologia Molecular - SBBq (33. 2004 Caxambu)

Programa e resumos Caxambu, MG

Caxambu 2004

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  • Título:
    Production of alkaline and acidic pectinases by Saccharomyces cerevisiae
  • Autor: K. F. Oliveira
  • C. S Souza; Elisabete José Vicente C. Laluce; Sociedade Brasileira de Bioquímica e Biologia Molecular - SBBq (33. 2004 Caxambu)
  • Assuntos: MICROBIOLOGIA
  • É parte de: Programa e resumos Caxambu, MG
  • Notas Locais: Disponível somente em CD-ROM
  • Descrição: Pectins are complex polysaccharides found in plant cell walls and fruit lamellae. Hydrolysis of pectin has been detected in cultures (supernatants) of Saccharomyces cerevisiae only at acidic pH values (Kashyap et al., Bioresour. Technol., 77:215, 2001). In the present work, Saccharomyces cerevisiae cells excreted acid and/or pectinolytic activities depending on the strain and the medium used. Four strains of yeast were propagated in media containing citrus pectin (as main carbon source), and low glucose concentration for stimulating growth. In fact, two types of culture media were used for the yeast propagation and they were a complex medium - containing peptone and yeast extract and minimal medium - containing yeast nitrogen base as the only nitrogen source. Pectinolytic activity (depolymerizing activity) was determined using high methoxylated citrus pectin. The excretion of alkaline pectinolytic activity was stimulated by growth in minimal medium, while acidic activities predominated in complex medium. High values of optimal temperatures (as high as 85_C) were shown by the both alkaline and acidic activities. E_ects of metals on pectin hydrolysis were dependent on the strain and growth conditions and they were detected as follows: no e_ect, activation or inhibition of the pectin hydrolysis obtained when EDTA (20 mM) or calcium ions (10 mM) were added to the assays. High thermal stabilities (as high as 70_C) were observed during the pre-incubation of
    supernatants for 120 minutes prior to enzymatic assays. Due to high pectinolytic activities obtained in a wide range of pH values, (pHs values as lower as 3.7 and or as high as 9.0), this organism has the possibility of acting as a live microbial feed supplement in the diet of animals as already suggested
  • Editor: Caxambu
  • Data de criação/publicação: 2004
  • Formato: res. L79.
  • Idioma: Inglês
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