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Different Methods for Seminal Plasma Removal and Sperm Selection on the Quality and Fertility of Collared Peccary Sperm

Santos, Maria V O ; Silva, Andréia M ; Aquino, Leonardo V C ; Oliveira, Lhara R M ; Moreira, Samara S J ; Oliveira, Moacir F ; Silva, Alexandre R ; Pereira, Alexsandra F

Animals (Basel), 2023-06, Vol.13 (12), p.1955 [Periódico revisado por pares]

Switzerland: MDPI AG

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Título:
Different Methods for Seminal Plasma Removal and Sperm Selection on the Quality and Fertility of Collared Peccary Sperm
Autor: Santos, Maria V O ; Silva, Andréia M ; Aquino, Leonardo V C ; Oliveira, Lhara R M ; Moreira, Samara S J ; Oliveira, Moacir F ; Silva, Alexandre R ; Pereira, Alexsandra F
Assuntos: Animals ; Centrifugation ; Comparative analysis ; Cryopreservation ; density gradient centrifugation ; Deoxyribonucleic acid ; DNA ; Embryos ; Experiments ; Fertility ; Gametocytes ; Hogs ; In vitro fertilization ; Integrity ; Methods ; Morphology ; Motility ; Oocytes ; Pecari tajacu ; Quality assessment ; Sample preparation ; Semen ; Sperm ; Spermatozoa ; Straight lines ; swim-up ; Swimming ; Swine ; Velocity ; Washing ; washing by centrifugation ; wild ungulates
É parte de: Animals (Basel), 2023-06, Vol.13 (12), p.1955
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Methods for seminal plasma (SP) removal and the selection of collared peccary sperm for fertilization were compared. The experiments evaluated the following: the (I) impact of centrifugation for SP removal before swim-up for sperm selection and (II) a comparison of different Percoll gradient densities (PG 45-90% and PG 35-70%). Non-selected sperm served as the control. Sperm quality was assessed based on motility patterns, morphology, membrane functional integrity, viability, reactive oxygen species (ROS), glutathione (GSH), and DNA integrity. Subsequently, the most successful group in the previous experiment and washing by centrifugation (WC) were compared for motility patterns and fertilization using pig oocytes. Swim-up decreased motility and enhanced ROS compared to the control. Centrifugation before swim-up harmed integrity and viability compared to the control. PG 45-90% (96.8 vs. 69.7 vs. 40.7 µm/s) allowed for a better velocity average pathway (VAP), a better velocity straight line, and better linearity (LIN) than those of the control and PG 35-70% (88.4 vs. 56.0 vs. 27.3 µm/s). Thus, PG 45-90% was used for fertilization. PG 45-90% obtained a higher VAP, a higher amplitude of the lateral head, straightness, and higher LIN than those of the control and WC. Cleavage (25.2-26.3%) and morula (8.1-10.5%) rates did not differ between the groups. Therefore, PG 45-90% and WC were efficient in isolating collared peccary sperm capable of fertilizing pig oocytes.
Editor: Switzerland: MDPI AG
Idioma: Inglês

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Different Methods for Seminal Plasma Removal and Sperm Selection on the Quality and Fertility of Collared Peccary Sperm

Santos, Maria V O ; Silva, Andréia M ; Aquino, Leonardo V C ; Oliveira, Lhara R M ; Moreira, Samara S J ; Oliveira, Moacir F ; Silva, Alexandre R ; Pereira, Alexsandra F

Switzerland: MDPI AG

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