Idioma:

Determination of antipsychotic drugs in oral fluid using dried saliva spots by gas chromatography-tandem mass spectrometry

Caramelo, Débora ; Rosado, Tiago ; Oliveira, Victor ; Rodilla, Jesus M. ; Rocha, Pedro M. M. ; Barroso, Mário ; Gallardo, Eugenia

Analytical and bioanalytical chemistry, 2019-09, Vol.411 (23), p.6141-6153 [Periódico revisado por pares]

Berlin/Heidelberg: Springer Berlin Heidelberg

Texto completo disponível

Citações Citado por

Enviar para

Título:
Determination of antipsychotic drugs in oral fluid using dried saliva spots by gas chromatography-tandem mass spectrometry
Autor: Caramelo, Débora ; Rosado, Tiago ; Oliveira, Victor ; Rodilla, Jesus M. ; Rocha, Pedro M. M. ; Barroso, Mário ; Gallardo, Eugenia
Assuntos: Accuracy ; Analysis ; Analytical Chemistry ; Antipsychotic drugs ; Antipsychotics ; Biochemistry ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Chlorpromazine ; Chromatography ; Clozapine ; Coefficient of variation ; Drugs ; Food Science ; Gas chromatography ; Haloperidol ; Ions ; Laboratory Medicine ; Linearity ; Mass spectrometry ; Mass spectroscopy ; Mathematical analysis ; Methods ; Monitoring/Environmental Analysis ; Optimization ; Psychotropic drugs ; Quetiapine ; Research Paper ; Saliva ; Scientific imaging ; Selectivity ; Sensitivity analysis ; Solvent extraction ; Spectroscopy
É parte de: Analytical and bioanalytical chemistry, 2019-09, Vol.411 (23), p.6141-6153
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: The present work describes the optimization and validation of an analytical method for the determination of six antipsychotic drugs (chlorpromazine, levomepromazine, cyamemazine, clozapine, haloperidol, and quetiapine) in oral fluid samples after solvent extraction from dried saliva spots, by gas chromatography coupled to tandem mass spectrometry. The method was fully validated, and the included parameters were selectivity, linearity, limits of quantification, precision and accuracy, stability, and recovery. The method was linear for all compounds from 10 to 400 ng/mL, except for haloperidol (5–100 ng/mL), presenting coefficients of determination higher than 0.99. Inter- and intra-day precision and accuracy were in conformity with the criteria usually seen in bioanalytical method validation; i.e., coefficients of variation were lower than 15% and an accuracy of 15% or better for all studied drugs. The recoveries obtained with this miniaturized technique ranged from 63 to 97%. The herein described method is the first to be reported using the dried saliva spots approach for the analysis of these antypshychotic drugs, proving great sensitivity apart from its simple and fast procedure. The method was considered a good alternative to the conventional techniques to be applied in clinical and toxicological analyses, even more taking into account the extremely low sample volume used (50 μL). Graphical abstract
Editor: Berlin/Heidelberg: Springer Berlin Heidelberg
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Determination of antipsychotic drugs in oral fluid using dried saliva spots by gas chromatography-tandem mass spectrometry

Caramelo, Débora ; Rosado, Tiago ; Oliveira, Victor ; Rodilla, Jesus M. ; Rocha, Pedro M. M. ; Barroso, Mário ; Gallardo, Eugenia

Berlin/Heidelberg: Springer Berlin Heidelberg

Buscando em bases de dados remotas. Favor aguardar.