Idioma:

Repeatability of gingival crevicular fluid collection and quantification, as determined through its alkaline phosphatase activity: implications for diagnostic use

Perinetti, G. ; Di Leonardo, B. ; Di Lenarda, R. ; Contardo, L.

Journal of periodontal research, 2013-02, Vol.48 (1), p.98-104 [Periódico revisado por pares]

Oxford, UK: Blackwell Publishing Ltd

Texto completo disponível

Citações Citado por

Enviar para

Título:
Repeatability of gingival crevicular fluid collection and quantification, as determined through its alkaline phosphatase activity: implications for diagnostic use
Autor: Perinetti, G. ; Di Leonardo, B. ; Di Lenarda, R. ; Contardo, L.
Assuntos: Adolescent ; Adult ; alkaline phosphatase ; Alkaline Phosphatase - analysis ; Biological and medical sciences ; Dental Plaque Index ; Dentistry ; diagnosis ; Female ; Follow-Up Studies ; Gingiva - secretion ; Gingival Crevicular Fluid - enzymology ; Gingival Crevicular Fluid - secretion ; Gingival Pocket - classification ; Humans ; Male ; Medical sciences ; method error ; Otorhinolaryngology. Stomatology ; Periodontal Index ; Periodontium - secretion ; Prospective Studies ; repeatability ; Reproducibility of Results ; resting/flow gingival crevicular fluid ; Single-Blind Method ; Specimen Handling - standards ; Spectrophotometry - methods ; Young Adult
É parte de: Journal of periodontal research, 2013-02, Vol.48 (1), p.98-104
Notas: ark:/67375/WNG-LWBXSKP2-P
istex:B7AC38BBB85A5EFC2F4E4AD0EE36E0132FBB9820
ArticleID:JRE1508
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Background and Objective: In spite of four decades of studies on gingival crevicular fluid, no data have been reported on the repeatability of gingival crevicular fluid collection and the subsequent quantification procedures. The present study reports, for the first time, on the repeatability and method error of gingival crevicular fluid collection and quantification, as determined through its alkaline phosphatase (ALP) activity. Diagnostic considerations are then explored. Material and Methods: Twenty‐seven healthy subjects (17 women and 10 men; mean age ± SD, 21.2 ± 4.8 years) with optimal periodontal status were enrolled according to a blind prospective design. The gingival crevicular fluid was collected at baseline, and after 1 d, 1 wk and 3 mo. At each clinical session, two consecutive rounds of gingival crevicular fluid collection were made from each of the four maxillary incisors, allowing the recovery of resting and flow gingival crevicular fluid. The total ALP activities were determined spectrophotometrically, and repeatability and method errors for the resting, flow and overall (resting + flow) gingival crevicular fluid ALP activities were calculated, relative to the corresponding baseline levels. Results: No significant differences were seen over time, although the flow gingival crevicular fluid ALP activity was generally lower than that for the resting gingival crevicular fluid. The method errors ranged from 40 to 58%, with the flow and overall gingival crevicular fluid activities showing the highest and lowest errors, respectively. Conclusion: Reliable use of the gingival crevicular fluid ALP collection and quantification, both in research and diagnosis on an individual basis, should take into account relevant errors, and variations are to be considered as true only above relevant thresholds.
Editor: Oxford, UK: Blackwell Publishing Ltd
Idioma: Inglês

Links

Voltar para lista de resultados

Anterior Resultado 7 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Repeatability of gingival crevicular fluid collection and quantification, as determined through its alkaline phosphatase activity: implications for diagnostic use

Perinetti, G. ; Di Leonardo, B. ; Di Lenarda, R. ; Contardo, L.

Oxford, UK: Blackwell Publishing Ltd

Buscando em bases de dados remotas. Favor aguardar.