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SoxB family genes delay regeneration and cause abnormal movement in Dugesia japonica

Yang, Yibo ; Lin, Ziyi ; Li, Nannan ; Li, Ning ; Liu, Dezeng ; Dong, Zimei ; Chen, Guangwen

Molecular genetics and genomics : MGG, 2024-12, Vol.299 (1), p.53-53, Article 53 [Periódico revisado por pares]

Berlin/Heidelberg: Springer Berlin Heidelberg

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Título:
SoxB family genes delay regeneration and cause abnormal movement in Dugesia japonica
Autor: Yang, Yibo ; Lin, Ziyi ; Li, Nannan ; Li, Ning ; Liu, Dezeng ; Dong, Zimei ; Chen, Guangwen
Assuntos: Amputation ; Animal Genetics and Genomics ; Animals ; Bacteria ; Biochemistry ; Biomedical and Life Sciences ; Cell proliferation ; Cell Proliferation - genetics ; Dugesia japonica ; E coli ; Genomics ; Helminth Proteins - genetics ; Helminth Proteins - metabolism ; Human Genetics ; Hybridization ; Life Sciences ; Microbial Genetics and Genomics ; Nerves ; Neurogenesis ; Original Article ; Phosphatase ; Planarians - genetics ; Planarians - physiology ; Plant Genetics and Genomics ; Proteins ; Regeneration ; Regeneration - genetics ; RNA Interference ; RNA-mediated interference ; SOXB1 Transcription Factors - genetics ; Stem cells ; Turbellaria ; Variance analysis ; Worms
É parte de: Molecular genetics and genomics : MGG, 2024-12, Vol.299 (1), p.53-53, Article 53
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: SoxB subfamily is an important branch of Sox family and plays a key role in animal physiological process, but little is known about their function in planarian regeneration. This study aims to evaluate the function of DjSoxB family genes in intact and regenerating planarians Dugesia japonica . Here, we amplify the full-length cDNA of DjSoxB1 and DjSoxB2 in D. japonica by rapid amplification of the cDNA ends (RACE), detect the expression of DjSoxB family genes in planarian. The results show that DjSoxBs are expressed in parenchymal tissue and the hybridization signals partially disappear after irradiation indicates DjSoxB family genes are expressed in neoblasts. After the RNA interference (RNAi) of DjSoxB1 , DjSoxB2 and DjSoxB3 separately, the numbers of proliferative cells are all reduced that causes planarians show slower growth of blastema in the early stage of regeneration, and nerves of planarians are affected that the movement speed of planarians decreases in varying degrees. Specially, planarians in the DjSoxB3 RNAi group show shrinkage and twisting. Overall, this study reveals that DjSoxB family genes play a role in cell proliferation during regeneration. They also play an important role in the maintenance of normal nerve function and nerve regeneration. These results provide directions for the functional study of SoxB family genes and provide an important foundation for planarian regeneration.
Editor: Berlin/Heidelberg: Springer Berlin Heidelberg
Idioma: Inglês

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SoxB family genes delay regeneration and cause abnormal movement in Dugesia japonica

Yang, Yibo ; Lin, Ziyi ; Li, Nannan ; Li, Ning ; Liu, Dezeng ; Dong, Zimei ; Chen, Guangwen

Berlin/Heidelberg: Springer Berlin Heidelberg

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