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Inhibitory function of adapter-related protein complex 2 alpha 1 subunit in the process of nuclear translocation of human immunodeficiency virus type 1 genome

Kitagawa, Yukiko ; Kameoka, Masanori ; Shoji-Kawata, Sanae ; Iwabu, Yukie ; Mizuta, Hiroyuki ; Tokunaga, Kenzo ; Fujino, Masato ; Natori, Yukikazu ; Yura, Yoshiaki ; Ikuta, Kazuyoshi

Virology (New York, N.Y.), 2008-03, Vol.373 (1), p.171-180 [Peer Reviewed Journal]

United States: Elsevier Inc

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Title:
Inhibitory function of adapter-related protein complex 2 alpha 1 subunit in the process of nuclear translocation of human immunodeficiency virus type 1 genome
Author: Kitagawa, Yukiko ; Kameoka, Masanori ; Shoji-Kawata, Sanae ; Iwabu, Yukie ; Mizuta, Hiroyuki ; Tokunaga, Kenzo ; Fujino, Masato ; Natori, Yukikazu ; Yura, Yoshiaki ; Ikuta, Kazuyoshi
Subjects: 60 APPLIED LIFE SCIENCES ; Active Transport, Cell Nucleus ; Adapter-related protein complex 2 alpha 1 subunit ; Adaptor Protein Complex 2 - genetics ; Adaptor Protein Complex 2 - metabolism ; ADSORPTION ; AIDS ; AIDS VIRUS ; ANIMAL CELLS ; AP-2 ; Cell Line ; Cell Nucleus - metabolism ; Cell Nucleus - virology ; CYTOPLASM ; Cytoplasm - metabolism ; DNA ; DNA, Viral - metabolism ; FLUORESCENCE ; GTP-ASES ; HIV-1 ; HIV-1 - genetics ; HIV-1 - pathogenicity ; HIV-1 - physiology ; Host factor ; Human immunodeficiency virus 1 ; Human immunodeficiency virus 2 ; Humans ; Infectious Disease ; Integration ; LIFE CYCLE ; Membrane Glycoproteins ; MICROSCOPY ; Nuclear translocation ; RNA ; RNA Interference ; RNA, Small Interfering - genetics ; RNA, Small Interfering - metabolism ; siRNA ; TRANSCRIPTION ; TRANSLOCATION ; Vesicular stomatitis virus ; Viral Envelope Proteins ; Virus Integration ; Virus Replication
Is Part Of: Virology (New York, N.Y.), 2008-03, Vol.373 (1), p.171-180
Notes: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Description: Abstract The transfection of human cells with siRNA against adapter-related protein complex 2 alpha 1 subunit (AP2α) was revealed to significantly up-regulate the replication of human immunodeficiency virus type 1 (HIV-1). This effect was confirmed by cell infection with vesicular stomatitis virus G protein-pseudotyped HIV-1 as well as CXCR4-tropic and CCR5-tropic HIV-1. Viral adsorption, viral entry and reverse transcription processes were not affected by cell transfection with siRNA against AP2α. In contrast, viral nuclear translocation as well as the integration process was significantly up-regulated in cells transfected with siRNA against AP2α. Confocal fluorescence microscopy revealed that a subpopulation of AP2α was not only localized in the cytoplasm but was also partly co-localized with lamin B, importin β and Nup153, implying that AP2α negatively regulates HIV-1 replication in the process of nuclear translocation of viral DNA in the cytoplasm or the perinuclear region. We propose that AP2α may be a novel target for disrupting HIV-1 replication in the early stage of the viral life cycle.
Publisher: United States: Elsevier Inc
Language: English

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Inhibitory function of adapter-related protein complex 2 alpha 1 subunit in the process of nuclear translocation of human immunodeficiency virus type 1 genome

Kitagawa, Yukiko ; Kameoka, Masanori ; Shoji-Kawata, Sanae ; Iwabu, Yukie ; Mizuta, Hiroyuki ; Tokunaga, Kenzo ; Fujino, Masato ; Natori, Yukikazu ; Yura, Yoshiaki ; Ikuta, Kazuyoshi

United States: Elsevier Inc

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