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Herpetomonas samuelpessoai: Electron microscopy and cytochemistry of electron-dense granules

de Carvalho, Técia Ulisses ; Souto-Padrón, Thais ; de Souza, Wanderley

Experimental parasitology, 1979-01, Vol.47 (3), p.297-304 [Periódico revisado por pares]

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Título:
Herpetomonas samuelpessoai: Electron microscopy and cytochemistry of electron-dense granules
Autor: de Carvalho, Técia Ulisses ; Souto-Padrón, Thais ; de Souza, Wanderley
Assuntos: Acid Phosphatase - analysis ; Animals ; arthropods ; Cytochemistry ; Cytoplasmic Granules - analysis ; Cytoplasmic Granules - enzymology ; Cytoplasmic Granules - ultrastructure ; Electron microscopy ; Electron-dense granules ; entomology ; Eukaryota - analysis ; Eukaryota - ultrastructure ; Herpetomonas samuelpessoai ; insects ; Iron - analysis ; parasitic ; Peroxidases - analysis ; Proteins - analysis ; Protozoa ; Trypanosomatidae
É parte de: Experimental parasitology, 1979-01, Vol.47 (3), p.297-304
Descrição: Herpetomonas samuelpessoai has membrane-bound electrondense granules in its cytoplasm. The electron density independs on postfixation with osmium tetroxide and is enhanced by uranyl acetate staining. The granules contain iron, have basic proteins cytochemically detected by the silver ammoniacal method, and have a peroxidase activity as detected by the diaminobenzidine method. Some of the granules also have acid phosphatase activity. It is suggested that the granules may represent either lysosomes or a storage form of tetrapyrrole derivatives which are essential for the growth and metabolism of most Trypanosomatidae.
Editor: United States: Elsevier Inc
Idioma: Inglês

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