skip to main content
Idioma:

Ancient bone collagen assessment by hand-held vibrational spectroscopy

Pestle, William J. ; Ahmad, Fakhra ; Vesper, Benjamin J. ; Cordell, Geoffrey A. ; Colvard, Michael D.

Journal of archaeological science, 2014-02, Vol.42, p.381-389 [Periódico revisado por pares]

Kidlington: Elsevier Ltd

Texto completo disponível

Citações Citado por
  • Título:
    Ancient bone collagen assessment by hand-held vibrational spectroscopy
  • Autor: Pestle, William J. ; Ahmad, Fakhra ; Vesper, Benjamin J. ; Cordell, Geoffrey A. ; Colvard, Michael D.
  • Assuntos: aDNA ; Collagen ; Excavation and methods ; FTIR ; Laboratory methods ; Methodology and general studies ; Prehistory and protohistory ; Radiocarbon ; Raman spectroscopy ; Stable isotope
  • É parte de: Journal of archaeological science, 2014-02, Vol.42, p.381-389
  • Descrição: Radiometric, isotopic, proteomic, and genetic studies of bone collagen are mainstays of archaeometric analysis. All four techniques are destructive and require substantial laboratory, temporal, and financial commitments. Because such analyses are predicated on the presence of a sufficient quantity of unaltered proteins (i.e. collagen), the development and validation of tools for the rapid, non-destructive, in situ analysis of collagen content could yield measurable benefits. In the present work, the results of a preliminary, proof-of-concept study on the utility of four hand-held vibrational spectroscopic instruments, one Fourier-Transform Infrared (FTIR) spectrometer and three Raman spectrometers (two with an excitation wavelength of 785 nm, and one with an excitation wavelength of 1030 nm), for analyzing the collagen content of archaeological bones are described. While the FTIR and 785 nm Raman devices showed little or no ability to discriminate between well- and poorly-preserved bone, the application of hand-held 1030 nm Raman spectroscopy appears to be well-suited for such a task. The ability to detect a measurable and characteristic spectroscopic peak associated with the δCH2 scissoring of Type I collagen in high-yielding, raw bone samples opens the door to the utilization of this technology in field research environments. •Collagen content in ancient bone assessed by hand-held FTIR and Raman.•Goal is identification of field-deployable tools for sample triage.•FTIR and 785 nm Raman devices do not produce useful results.•1030 nm Raman differentiates between well- and poorly preserved collagen.
  • Editor: Kidlington: Elsevier Ltd
  • Idioma: Inglês
Links
Voltar para lista de resultados
Ir para página anteriorAnterior Resultado  3 AvançarIr para próxima página

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.