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In vivo effects of GnRH on expression of interleukin 1 (IL-1) system members in bovine preovulatory follicles and the influence of IL-1β on cumulus-oocyte complexes cultured in vitro

Silva, A.W.B. ; Passos, J.R.S. ; Dau, A.M.P. ; De Cesaro, M.P. ; Ferst, J.G. ; dos Santos, J. Tonellotto ; Bezerra, F.T.G. ; Costa, J.J.N. ; Paulino, L.R.F.M. ; Donato, M.A.M. ; Peixoto, C.A. ; Gonçalves, P.B.D. ; Silva, J.R.V.

Livestock science, 2017-12, Vol.206, p.166-174 [Periódico revisado por pares]

Elsevier B.V

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  • Título:
    In vivo effects of GnRH on expression of interleukin 1 (IL-1) system members in bovine preovulatory follicles and the influence of IL-1β on cumulus-oocyte complexes cultured in vitro
  • Autor: Silva, A.W.B. ; Passos, J.R.S. ; Dau, A.M.P. ; De Cesaro, M.P. ; Ferst, J.G. ; dos Santos, J. Tonellotto ; Bezerra, F.T.G. ; Costa, J.J.N. ; Paulino, L.R.F.M. ; Donato, M.A.M. ; Peixoto, C.A. ; Gonçalves, P.B.D. ; Silva, J.R.V.
  • Assuntos: Antral follicle ; Cattle ; Cytokine ; Ovary ; Ovulation
  • É parte de: Livestock science, 2017-12, Vol.206, p.166-174
  • Descrição: The purpose of this study was to determine the effects of in vivo GnRH treatment on mRNA expression of IL-1β system in bovine granulosa cells from preovulatory follicles and to analyze the in vitro effect of gonadotropins on the IL-1β system gene expression in cumulus cells. Moreover, the additive effect of IL-1β on cumulus expansion, gene expression and ultrastructural integrity of COCs cultured in vitro was evaluated. In vivo studies in granulosa cells demonstrated that IL-1RA mRNA levels were increased after 24h of GnRH treatment. Similarly, the presence of gonadotropins increased the levels of mRNAs for IL-1R1 and IL-1RA in cumulus cells after 24h of culture in vitro. The presence of IL-1β in culture medium did not influence cumulus expansion, but it increased the expression of mRNAs for IL-1β, IL-1RA, HAS2 and iNOS after COC culture. In addition, the ultrastructure of COCs cultured in vitro in the presence of IL-1β was maintained. In conclusion, IL-1RA mRNA is upregulated in granulosa cells at later stages of the ovulatory process in cattle. In cumulus cells, mRNA expression of IL-1RI and IL-1RA is induced by gonadotropins and IL-1β maintains the ultrastructure of COC cultured in vitro. •IL-1RA mRNA is upregulated in bovine granulosa cells after in vivo GnRH treatment.•mRNA expression of IL-1RI and IL-1RA is induced by gonadotropins in cultured cumulus cells.•IL-1β increases iNOS mRNA in cultured cumulus cells in a time-dependent manner.•IL-1β maintains the ultrastructure of COC cultured in vitro.
  • Editor: Elsevier B.V
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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