skip to main content
Guest
e-Shelf
My Account
Sign out
Sign in
This feature requires javascript
Tags
e-Journals
e-Books
Databases
USP Libraries
Help
Help
Language:
English
Spanish
Portuguese (Brazil)
This feature required javascript
This feature requires javascript
Primo Advanced Search
General Search
General Search
Physical Collection
Physical Collections
USP Intelectual Production
USP Production
Primo Advanced Search Query Term
Input search text:
Show Results with:
criteria input
Any
Show Results with:
Any
Primo Advanced Search prefilters
Material Type:
criteria input
All items
General Search
Simple Search
This feature requires javascript
10 TIMED ARTIFICIAL INSEMINATION IN CATTLE WITH REDUCED DOSAGE OF SPERMATOZOA
Becker, F. ; Alm, H. ; Schneider, F. ; Nehring, H. ; Rothe, L. ; Kanitz, W.
Reproduction fertility and development, 2005, Vol.17 (2), p.155
[Peer Reviewed Journal]
Full text available
Citations
Cited by
View Online
Details
Reviews & Tags
More
Times Cited
This feature requires javascript
Actions
Add to e-Shelf
Remove from e-Shelf
E-mail
Print
Permalink
Citation
EasyBib
EndNote
RefWorks
Delicious
Export RIS
Export BibTeX
This feature requires javascript
Title:
10 TIMED ARTIFICIAL INSEMINATION IN CATTLE WITH REDUCED DOSAGE OF SPERMATOZOA
Author:
Becker, F.
;
Alm, H.
;
Schneider, F.
;
Nehring, H.
;
Rothe, L.
;
Kanitz, W.
Is Part Of:
Reproduction fertility and development, 2005, Vol.17 (2), p.155
Description:
Estrus detection and determination of time of insemination are very important factors in reproduction management of cattle. Therefore an estrus synchronization schedule in combination with induction of ovulation and a single insemination at a predetermined time in dairy cattle was established to achieve high pregnancy rates (Kanitz etal. 2002 Reprod. Nutr. Dev. 42, 587–599; Becker et al. 2004). The aim of the recent study was to investigate the influence of the number of spermatozoa per insemination dosage on embryo development and the interrelationship between number of accessory sperms per embryo and its development using this schedule. In total 116 German Holstein heifers received GnRH (i.m.; 0.05 mg Gonavet®, Veyx, Schwarzenborn, Germany) 60 h after PGF2α application (i.m.; 0.5 mg Cloprostenol forte®, Jenapharm, Jena, Germany) administered between Days 8 and 14 of the estrous cycle. Artificial insemination was carried out 13 h after GnRH application. Three different dosages of spermatozoa (15 × 106, 5 × 106, and 1 × 106) from three ejaculates from four fertile bulls were used. Embryos and oocytes were flushed from the oviducts of animals ovulated (n = 106; ovulation rate 91.3%). Animals were slaughtered on Day 4 after insemination. The quality of the embryos and oocytes was evaluated by microscopic examination. Embryos were stained with Hoechst 33258 to verify the number of accessory sperm. The evaluation of the data was carried out with the GLM procedure of the statistics software package SAS® (SAS Institute, Inc., Cary, NC, USA). As a post-hoc test the Student’s t-test was used. Significance was set at P = 0.05. After flushing of 106 animals, 85 embryos and oocytes was recovered (recovery rate 80.2%). Relative to the three sperm concentrations, there were no significant differences among fertilization rates (92.3, 96.2, and 78.8%) and among the portions of normal developed embryos (84.6, 80.7, and 75.8%, all respectively) between groups. Interestingly, significant differences were found according to the mean number of accessory sperm/embryo (29.6 ± 8.4, 45.3 ± 8.6, and 6.5 ± 7.2, respectively) and in the portion of embryos without or with more than 10 accessory sperm/embryo. Results show that fixed-time insemination, independent of detection of onset of estrus can result in high fertilization rates. Insemination with dosages <5 × 106 spermatozoa can reduce fertilization rates. Likewise, significant differences regarding fertilization rate were found after insemination of reduced sperm dosages of individual bulls. The number of accessory sperms/embryo seems to be an irrelevant parameter for quality of embryos produced under described conditions.
Language:
English
This feature requires javascript
This feature requires javascript
Back to results list
Previous
Result
8
Next
This feature requires javascript
This feature requires javascript
Searching Remote Databases, Please Wait
Searching for
in
scope:(USP_VIDEOS),scope:("PRIMO"),scope:(USP_FISICO),scope:(USP_EREVISTAS),scope:(USP),scope:(USP_EBOOKS),scope:(USP_PRODUCAO),primo_central_multiple_fe
Show me what you have so far
This feature requires javascript
This feature requires javascript