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A Simplified System for Generating Recombinant Adenoviruses

He, Tong-Chuan ; Zhou, Shibin ; Da Costa, Luis T. ; Yu, Jian ; Kinzler, Kenneth W. ; Vogelstein, Bert

Proceedings of the National Academy of Sciences - PNAS, 1998-03, Vol.95 (5), p.2509-2514 [Periódico revisado por pares]

United States: National Academy of Sciences of the United States of America

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Título:
A Simplified System for Generating Recombinant Adenoviruses
Autor: He, Tong-Chuan ; Zhou, Shibin ; Da Costa, Luis T. ; Yu, Jian ; Kinzler, Kenneth W. ; Vogelstein, Bert
Assuntos: Adenoviridae - genetics ; Adenoviruses ; Animals ; Biological Sciences ; Cell Line ; Cell lines ; Escherichia coli - genetics ; Genes ; Genes, Reporter ; Genetic Vectors ; Green Fluorescent Proteins ; HEK293 cells ; Homologous recombination ; Humans ; Kidney ; Luminescent Proteins - biosynthesis ; Mammals ; Medical research ; Packaging ; Plasmids ; Polymerase Chain Reaction ; Recombinant Fusion Proteins - biosynthesis ; Recombination, Genetic ; Repetitive Sequences, Nucleic Acid ; Transfection ; Transfection - methods ; Transgenes ; Viruses
É parte de: Proceedings of the National Academy of Sciences - PNAS, 1998-03, Vol.95 (5), p.2509-2514
Notas: ObjectType-Article-2
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To whom reprint requests should be addressed. e-mail: tche@welchlink.welch.jhu.edu.
Contributed by Bert Vogelstein
Descrição: Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. We report herein a strategy that simplifies the generation and production of such viruses. A recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulations, using homologous recombination in bacteria rather than in eukaryotic cells. After transfections of such plasmids into a mammalian packaging cell line, viral production is conveniently followed with the aid of green fluorescent protein, encoded by a gene incorporated into the viral backbone. Homogeneous viruses can be obtained from this procedure without plaque purification. This system should expedite the process of generating and testing recombinant adenoviruses for a variety of purposes.
Editor: United States: National Academy of Sciences of the United States of America
Idioma: Inglês

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A Simplified System for Generating Recombinant Adenoviruses

He, Tong-Chuan ; Zhou, Shibin ; Da Costa, Luis T. ; Yu, Jian ; Kinzler, Kenneth W. ; Vogelstein, Bert

United States: National Academy of Sciences of the United States of America

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