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Inorganic pyrophosphatase from E. coli as a label for the detection of plant viruses by ELISA

Mizenina, O.A. (Moscow State Univ. (USSR). Dept. of Virology) ; Borisova, O.V ; Novikov, V.K ; Evtushenko, O.A ; Baykov, A.A ; Atabekov, J.G

Journal of phytopathology, 1991-12, Vol.133 (4), p.278-288 [Periódico revisado por pares]

Oxford, UK: Blackwell Publishing Ltd

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Título:
Inorganic pyrophosphatase from E. coli as a label for the detection of plant viruses by ELISA
Autor: Mizenina, O.A. (Moscow State Univ. (USSR). Dept. of Virology) ; Borisova, O.V ; Novikov, V.K ; Evtushenko, O.A ; Baykov, A.A ; Atabekov, J.G
Assuntos: Agronomy. Soil science and plant productions ; Bacteria ; Biological and medical sciences ; ELISA ; ENZIMAS ; Enzym ; ENZYME ; Escherichia coli ; Fundamental and applied biological sciences. Psychology ; Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control ; IDENTIFICACION ; IDENTIFICATION ; Identifizierung ; METHODE ; Methodik ; METODOS ; Phytopathology. Animal pests. Plant and forest protection ; Plant viruses and viroids ; Serologie ; TEST ELISA ; UdSSR ; Virus ; VIRUS DE LAS PLANTAS ; VIRUS DES VEGETAUX
É parte de: Journal of phytopathology, 1991-12, Vol.133 (4), p.278-288
Notas: 92U0083
H20
istex:B5B2F7DBE2E314E1131D3CF6F7E8A76D4586B2C5
ArticleID:JPH278
ark:/67375/WNG-PV3WGR2Q-X
With 6 figures
Descrição: Inorganic pyrophosphatase (PPase) from Escherichia coli was used as label enzyme immunoassay for detection of different plant viruses. The use of PPase-conjugated antibodies and tetrasodium pyrophosphate as a substrate in ELISA provides some advantages in detection of plant viruses in purified preparations and extracts from plant specimens: high sensitivity, negligible level of background reactions in control samples, bright blue-greenish colour which allows to detect the reaction visually and high stability of PPase-conjugated antibodies
Editor: Oxford, UK: Blackwell Publishing Ltd
Idioma: Inglês

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Inorganic pyrophosphatase from E. coli as a label for the detection of plant viruses by ELISA

Mizenina, O.A. (Moscow State Univ. (USSR). Dept. of Virology) ; Borisova, O.V ; Novikov, V.K ; Evtushenko, O.A ; Baykov, A.A ; Atabekov, J.G

Oxford, UK: Blackwell Publishing Ltd

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