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Gene expression by the cold-water octocoral Dentomuricea aff. meteor during an ex-situ experiment testing the effects of mining-generated sediment plumes

Carreiro-Silva, Marina ; Martins, Ines ; Raimundo, Joana ; Caetano, Miguel ; Bettencourt, Raul ; Cerqueira, Teresa ; Colaço, Ana

PANGAEA 2022

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Título:
Gene expression by the cold-water octocoral Dentomuricea aff. meteor during an ex-situ experiment testing the effects of mining-generated sediment plumes
Autor: Carreiro-Silva, Marina ; Martins, Ines ; Raimundo, Joana ; Caetano, Miguel ; Bettencourt, Raul ; Cerqueira, Teresa ; Colaço, Ana
Assuntos: A Trans-Atlantic assessment and deep-water ecosystem-based spatial management plan for Europe (ATLAS) ; Cnidaria ; Cycles ; enzyme activity ; experiment ; Experiment duration ; Experimental treatment ; gene expression ; Gene expression, fold change, relative ; Gene expression, fold change, relative, standard error ; Gene name ; Integrated Assessment of Atlantic Marine Ecosystems in Space and Time (iAtlantic) ; JPI Oceans - Ecological Aspects of Deep-Sea Mining (JPIO-MiningImpact) ; Managing Impacts of Deep-seA reSource exploitation (MIDAS) ; metabolism ; metal bioaccumulation ; North Atlantic ; octocorals ; Real-Time PCR, CFX Connect™ Real-Time PCR Detection System (BioRad) ; Specimen identification
Notas: RelationTypeNote: References -- 10.3389/fmars.2022.915650
10.1594/pangaea.948414
10.3389/fmars.2022.915650
RelationTypeNote: IsPartOf -- 10.1594/pangaea.948414
Gene expression profiles in D. aff. meteor exposed to the different treatmentsHKG: House Keeping Genes (18S and β-actin)RHD: rel homology domain TLR: toll-like receptor HSP70: heat shock protein RP Tyr-PH: receptor-type protein tyrosine phosphatase SOD: superoxide dismutaseCarb Anhy: α-carbonic anhydraseCt = cycle threshold (nº of cycles in an RT-PCR required for the signal to exceed the background level)Relative gene expression of the gene = Relative quantity gene / Geometric mean of the relative quantities of each housekeeping gene (=RQ gene/Geomean RQ HKGs)Relative quantity (RQ) = (efficiency of the gene)^ΔCT
Descrição: We report the results of an aquaria-based experiment testing the effects of suspended particles generated during potential mining activities, on a common habitat-building coral species in the Azores, Dentomuricea aff. meteor. Coral fragments were maintained in 10-L aquaria and exposed to three experimental treatments for a period of four weeks at the DeepSeaLab aquaria facilities (Okeanos-University of the Azores): (1) control conditions (no added sediments); (2) suspended polymetallic sulphide (PMS) particles; (3) suspended quartz particles. Gene expression profiles in D. aff. meteor were used to evaluate the physiological pathways involved in the response to exposure to PMS and quartz particles. Coral fragments were collected from each treatment at times 0, 3, and 13 days and for the control and quartz treatments also at time 27 days. The study targeted genes involved in cellular stress and antioxidant reaction system (heat shock protein, superoxide dismutase, ferritin), cell structure/integrity (α-carbonic anhydrase, receptor-type protein tyrosine phosphatase) and immune responses (toll-like receptor, lysozyme, rel homology domain, ferritin).
Editor: PANGAEA
Data de criação/publicação: 2022
Idioma: Inglês

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Gene expression by the cold-water octocoral Dentomuricea aff. meteor during an ex-situ experiment testing the effects of mining-generated sediment plumes

Carreiro-Silva, Marina ; Martins, Ines ; Raimundo, Joana ; Caetano, Miguel ; Bettencourt, Raul ; Cerqueira, Teresa ; Colaço, Ana

PANGAEA 2022

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