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Quercitrin Nanocoated Implant Surfaces Reduce Osteoclast Activity In Vitro and In Vivo

Córdoba, Alba ; Manzanaro-Moreno, Nahuel ; Colom, Carme ; Rønold, Hans J ; Lyngstadaas, Staale P ; Monjo, Marta ; Ramis, Joana M

International journal of molecular sciences, 2018-10, Vol.19 (11), p.3319 [Periódico revisado por pares]

Switzerland: MDPI AG

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Título:
Quercitrin Nanocoated Implant Surfaces Reduce Osteoclast Activity In Vitro and In Vivo
Autor: Córdoba, Alba ; Manzanaro-Moreno, Nahuel ; Colom, Carme ; Rønold, Hans J ; Lyngstadaas, Staale P ; Monjo, Marta ; Ramis, Joana M
Assuntos: Acid phosphatase ; Acid phosphatase (tartrate-resistant) ; Acid resistance ; Adenosine triphosphatase ; Alkaline phosphatase ; Alkaline Phosphatase - metabolism ; animal experiments ; Animals ; Biocompatibility ; Biocompatible Materials - chemistry ; Biocompatible Materials - pharmacology ; Biological activity ; biomaterials ; Biomedical materials ; Bisphosphonates ; bone implant interactions ; Bone implants ; Bone resorption ; Cell culture ; Control surfaces ; Cytotoxicity ; Dental implants ; Dental prosthetics ; Failure ; Female ; Fibroblasts ; Flavonoids ; Gelatinase B ; Gene expression ; Growth factors ; In vivo methods and tests ; L-Lactate dehydrogenase ; L-Lactate Dehydrogenase - metabolism ; Lactate dehydrogenase ; Lactic acid ; Mice ; Osseointegration - drug effects ; Osteoclastogenesis ; Osteoclasts - drug effects ; Osteoclasts - metabolism ; Osteogenesis - drug effects ; Osteoporosis ; Phosphatase ; polyphenols ; Polyphenols - metabolism ; Prostheses and Implants ; Quercetin - analogs & derivatives ; Quercetin - chemistry ; Rabbits ; RANK Ligand - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Stem cells ; surface chemistry ; Surgical implants ; Tartrate-Resistant Acid Phosphatase - metabolism ; Titanium ; TRANCE protein ; Wounds
É parte de: International journal of molecular sciences, 2018-10, Vol.19 (11), p.3319
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Descrição: In this study, the effect on osteoclast activity in vitro and in vivo of titanium implants that were coated with quercitrin was evaluated. Titanium surfaces were covalently coated with the flavonoid quercitrin. The effect of the surfaces on osteoclastogenesis was first tested in vitro on RAW264.7 cells that were supplemented with receptor activator of nuclear factor kappa-B ligand (RANKL) to generate osteoclast-like cells by tartrate-resistant acid phosphatase (TRAP) inmunostaining after five days of culture, and by analysis of the mRNA expression levels of markers related to bone resorption after seven days of culture. A rabbit tibial model was used to evaluate the in vivo biological response to the implant surfaces after eight weeks of healing, analyzing the lactate dehydrogenase (LDH) and the alkaline phosphatase (ALP) activities in the wound fluid that were present at the implant interface and the peri-implant bone mRNA expression levels of several markers related to inflammation, bone resorption and osteoblast-osteoclast interaction. No differences between groups and control surfaces were found in the wound fluid analyses. Moreover, quercitrin implant surfaces significantly decreased the expression of osteoclast related genes in vitro ( , , , , ) and in vivo ( , , ) as well as the expression of in vivo. Moreover, quercitrin surfaces were not cytotoxic for the cells. Thus, quercitrin implant surfaces were biocompatible and decreased osteoclastogenesis in vitro and in vivo. This could be used to improve the performance of dental implants.
Editor: Switzerland: MDPI AG
Idioma: Inglês;Norueguês

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Quercitrin Nanocoated Implant Surfaces Reduce Osteoclast Activity In Vitro and In Vivo

Córdoba, Alba ; Manzanaro-Moreno, Nahuel ; Colom, Carme ; Rønold, Hans J ; Lyngstadaas, Staale P ; Monjo, Marta ; Ramis, Joana M

Switzerland: MDPI AG

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