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The Influence of Silver Nanoparticles Against Toxic Effects of Philodryas olfersii Venom

Proença-Assunção, Jaqueline de Cássia ; Farias-de-França, Anna Paula ; Tribuiani, Natalia ; Cogo, Jose Carlos ; Collaço, Rita de Cássia ; Randazzo-Moura, Priscila ; Consonni, Sílvio Roberto ; Chaud, Marco Vinicius ; Dos Santos, Carolina Alves ; Oshima-Franco, Yoko

International journal of nanomedicine, 2021-01, Vol.16, p.3555-3564 [Periódico revisado por pares]

New Zealand: Dove Medical Press Limited

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Título:
The Influence of Silver Nanoparticles Against Toxic Effects of Philodryas olfersii Venom
Autor: Proença-Assunção, Jaqueline de Cássia ; Farias-de-França, Anna Paula ; Tribuiani, Natalia ; Cogo, Jose Carlos ; Collaço, Rita de Cássia ; Randazzo-Moura, Priscila ; Consonni, Sílvio Roberto ; Chaud, Marco Vinicius ; Dos Santos, Carolina Alves ; Oshima-Franco, Yoko
Assuntos: Acetylcholine ; Analysis ; Animals ; chick biventer cervicis ; Chickens ; Colubridae ; Creatine ; Creatine kinase ; Creatine Kinase - metabolism ; Curcumin - chemistry ; Dose-Response Relationship, Drug ; Edema ; Experiments ; Laboratory animals ; Male ; Medical research ; Metal Nanoparticles ; Microscopy ; Muscle Contraction - drug effects ; Muscle, Skeletal - drug effects ; Nanoparticles ; neuromuscular blockade ; Neurotoxins - antagonists & inhibitors ; Neurotoxins - toxicity ; opisthoglyphous snakes ; Original Research ; philodryas olfersii ; Phrenic Nerve - drug effects ; Rats ; Silver ; Silver - chemistry ; Silver - pharmacology ; silver nanoparticles ; Snake Venoms - antagonists & inhibitors ; Snake Venoms - toxicity ; Snakes ; Venom
É parte de: International journal of nanomedicine, 2021-01, Vol.16, p.3555-3564
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: A silver nanoparticle obtained by reducing salts with solid dispersion of curcumin (130 nm, 0.081 mg mL ) was used to counteract against the toxic - edematogenic, myotoxic, and neurotoxic - effects of venom. The edematogenic effect was evaluated by plasma extravasation in rat dorsal skin after injection of 50 µg per site of venom alone or preincubated with 1, 10, and 100 µL of AgNPs; the myotoxicity was evaluated by measuring the creatine kinase released into the organ-bath before the treatment and at the end of each experiment; and neurotoxicity was evaluated in chick biventer cervicis using the conventional myographic technique, face to the exogenous acetylcholine (ACh) and potassium chloride (KCl) added into the bath before the treatment and after each experiment. Preliminarily, a concentration-response curve of AgNPs was carried out to select the concentration to be used for neutralizing assays, which consists of neutralizing the venom-induced neuromuscular paralysis and edema by preincubating AgNPs with venom for 30 min. The venom-induced edema (n=6) and a complete neuromuscular blockade (n=4) that includes the total and unrecovered block of ACh and KCl contractures. AgNPs produced a concentration-dependent decrease the venom-induced edema (n=6) from 223.3% to 134.4% and to 100.5% after 10 and 100 µL AgNPs-preincubation, respectively. The preincubation of venom with AgNPs (1 µL; n=6) was able to maintain 46.5 ± 10.9% of neuromuscular response under indirect stimuli, 39.2 ± 9.7% of extrinsic nicotinic receptors functioning in absence of electrical stimulus and 28.3 ± 8.1% of responsiveness to potassium on the sarcolemmal membrane. The CK release was not affected by any experimental protocol which was like control. AgNPs interact with constituents of venom responsible for the edema-forming activity and neuromuscular blockade, but not on the sarcolemma membrane-acting constituents. The protective effect of the studied AgNPs on avian preparation points out to molecular targets as intrinsic and extrinsic nicotinic receptors.
Editor: New Zealand: Dove Medical Press Limited
Idioma: Inglês

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The Influence of Silver Nanoparticles Against Toxic Effects of Philodryas olfersii Venom

Proença-Assunção, Jaqueline de Cássia ; Farias-de-França, Anna Paula ; Tribuiani, Natalia ; Cogo, Jose Carlos ; Collaço, Rita de Cássia ; Randazzo-Moura, Priscila ; Consonni, Sílvio Roberto ; Chaud, Marco Vinicius ; Dos Santos, Carolina Alves ; Oshima-Franco, Yoko

New Zealand: Dove Medical Press Limited

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