Idioma:

A microfluidics-based in vitro model of the gastrointestinal human-microbe interface

Shah, Pranjul ; Fritz, Joëlle V ; Glaab, Enrico ; Desai, Mahesh S ; Greenhalgh, Kacy ; Frachet, Audrey ; Niegowska, Magdalena ; Estes, Matthew ; Jäger, Christian ; Seguin-Devaux, Carole ; Zenhausern, Frederic ; Wilmes, Paul

Nature communications, 2016-05, Vol.7 (1), p.11535-11535, Article 11535 [Revista revisada por pares]

England: Nature Publishing Group

Texto completo disponible

Citas Citado por

Acciones

Título:
A microfluidics-based in vitro model of the gastrointestinal human-microbe interface
Autor: Shah, Pranjul ; Fritz, Joëlle V ; Glaab, Enrico ; Desai, Mahesh S ; Greenhalgh, Kacy ; Frachet, Audrey ; Niegowska, Magdalena ; Estes, Matthew ; Jäger, Christian ; Seguin-Devaux, Carole ; Zenhausern, Frederic ; Wilmes, Paul
Materias: Aerobiosis ; Anaerobiosis ; Bacteria - cytology ; Caco-2 Cells ; Cell culture ; Coculture Techniques ; Gastrointestinal Microbiome ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Humans ; Infections ; Interfaces ; Metabolomics ; Microfluidics - methods ; Microorganisms ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Models, Biological ; Reproducibility of Results
Es parte de: Nature communications, 2016-05, Vol.7 (1), p.11535-11535, Article 11535
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Present address: Department of Infection and Immunity, Luxembourg Institute of Health, 29 rue Henri Koch, L-4354 Esch-sur-Alzette, Luxembourg
Descripción: Changes in the human gastrointestinal microbiome are associated with several diseases. To infer causality, experiments in representative models are essential, but widely used animal models exhibit limitations. Here we present a modular, microfluidics-based model (HuMiX, human-microbial crosstalk), which allows co-culture of human and microbial cells under conditions representative of the gastrointestinal human-microbe interface. We demonstrate the ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions. In addition, we show that the co-culture of human epithelial cells with the obligate anaerobe Bacteroides caccae and LGG results in a transcriptional response, which is distinct from that of a co-culture solely comprising LGG. HuMiX facilitates investigations of host-microbe molecular interactions and provides insights into a range of fundamental research questions linking the gastrointestinal microbiome to human health and disease.
Editor: England: Nature Publishing Group
Idioma: Inglés

Enlaces

View this record in MEDLINE/PubMed

Volver a la lista de resultados

Resultado 1 Siguiente Ir a la siguiente página

Realización: Logos de Redes Sociales:

A microfluidics-based in vitro model of the gastrointestinal human-microbe interface

Shah, Pranjul ; Fritz, Joëlle V ; Glaab, Enrico ; Desai, Mahesh S ; Greenhalgh, Kacy ; Frachet, Audrey ; Niegowska, Magdalena ; Estes, Matthew ; Jäger, Christian ; Seguin-Devaux, Carole ; Zenhausern, Frederic ; Wilmes, Paul

England: Nature Publishing Group

Buscando en bases de datos remotas, por favor espere