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Fluorescent Nanocomposite Hydrogels Based on Conjugated Polymer Nanoparticles as Platforms for Alkaline Phosphatase Detection

Alacid, Yolanda ; Esquembre, Rocío ; Montilla, Francisco ; Martínez-Tomé, María José ; Mateo, C Reyes

Biosensors (Basel), 2023-03, Vol.13 (3), p.408 [Periódico revisado por pares]

Switzerland: MDPI AG

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Título:
Fluorescent Nanocomposite Hydrogels Based on Conjugated Polymer Nanoparticles as Platforms for Alkaline Phosphatase Detection
Autor: Alacid, Yolanda ; Esquembre, Rocío ; Montilla, Francisco ; Martínez-Tomé, María José ; Mateo, C Reyes
Assuntos: Alkaline Phosphatase ; Biocompatibility ; Carbon ; Enzymatic activity ; Enzyme activity ; Enzymes ; Fluorescence ; fluorescent sensor ; Graphene ; Homogeneity ; Hydrogels ; immobilization ; Life sciences ; nanocomposite hydrogel ; Nanocomposites ; Nanogels ; Nanomaterials ; Nanoparticles ; Phosphatase ; Phosphatases ; Physiological aspects ; Polyacrylamide ; polyfluorene ; Polymer industry ; Polymerization ; Polymers ; portable device ; Quantum dots ; Reproducibility of Results ; Sensors ; Shelf life ; Storage stability ; Thiadiazoles ; Xerogels
É parte de: Biosensors (Basel), 2023-03, Vol.13 (3), p.408
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: This work describes the development and characterization of fluorescent nanocomposite hydrogels, with high swelling and absorption capacity, and prepared using a green protocol. These fluorescent materials are obtained by incorporating, for the first time, polyfluorenes-based nanoparticles with different emission bands-poly[9,9-dioctylfluorenyl-2,7-diyl] (PFO) and poly[(9,9-di-n-octylfluorenyl-2,7-diyl)-alt-(1,4-benzo-{2,1,3}-thiadiazole)] (F8BT)-into a three-dimensional polymeric network based on polyacrylamide. To this end, two strategies were explored: incorporation of the nanoparticles during the polymerization process (in situ) and embedment after the hydrogel formation (ex situ). The results show that the combination of PFO nanoparticles introduced by the ex situ method provided materials with good storage stability, homogeneity and reproducibility properties, allowing their preservation in the form of xerogel. The fluorescent nanocomposite hydrogels have been tested as a transportable and user-friendly sensing platform. In particular, the ability of these materials to specifically detect the enzyme alkaline phosphatase (ALP) has been evaluated as a proof-of-concept. The sensor was able to quantify the presence of the enzyme in an aqueous sample with a response time of 10 min and LOD of 21 nM. Given these results, we consider that this device shows great potential for quantifying physiological ALP levels as well as enzyme activity in environmental samples.
Editor: Switzerland: MDPI AG
Idioma: Inglês

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Fluorescent Nanocomposite Hydrogels Based on Conjugated Polymer Nanoparticles as Platforms for Alkaline Phosphatase Detection

Alacid, Yolanda ; Esquembre, Rocío ; Montilla, Francisco ; Martínez-Tomé, María José ; Mateo, C Reyes

Switzerland: MDPI AG

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