Idioma:

Myosin XVI Regulates Actin Cytoskeleton Dynamics in Dendritic Spines of Purkinje Cells and Affects Presynaptic Organization

Roesler, Mona Katrin ; Lombino, Franco Luis ; Freitag, Sandra ; Schweizer, Michaela ; Hermans-Borgmeyer, Irm ; Schwarz, Jürgen R ; Kneussel, Matthias ; Wagner, Wolfgang

Frontiers in cellular neuroscience, 2019-08, Vol.13, p.330-330 [Periódico revisado por pares]

Switzerland: Frontiers Research Foundation

Texto completo disponível

Citações Citado por

Enviar para

Título:
Myosin XVI Regulates Actin Cytoskeleton Dynamics in Dendritic Spines of Purkinje Cells and Affects Presynaptic Organization
Autor: Roesler, Mona Katrin ; Lombino, Franco Luis ; Freitag, Sandra ; Schweizer, Michaela ; Hermans-Borgmeyer, Irm ; Schwarz, Jürgen R ; Kneussel, Matthias ; Wagner, Wolfgang
Assuntos: Actin ; actin cytoskeleton ; Autism ; autism spectrum disorder ; Cerebellum ; Cognition ; Cytoskeleton ; dendritic spine ; Dendritic spines ; Filaments ; Fluorescence recovery after photobleaching ; Mental disorders ; Microscopy ; Motor ability ; Motor skill learning ; Myo16 ; Myosin ; Nervous system diseases ; Neurobiology ; Neurons ; Neuroscience ; Neurosciences ; Photobleaching ; Polymerization ; Proteins ; Purkinje cell ; Purkinje cells ; Regulation ; Social discrimination learning ; Social interactions ; Synaptic vesicles ; Vocalization behavior ; WAVE
É parte de: Frontiers in cellular neuroscience, 2019-08, Vol.13, p.330-330
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Reviewed by: Maurizio Giustetto, University of Turin, Italy; Archan Ganguly, University of California, San Diego, United States
This article was submitted to Cellular Neurophysiology, a section of the journal Frontiers in Cellular Neuroscience
Edited by: João Peça, University of Coimbra, Portugal
Descrição: The actin cytoskeleton is crucial for function and morphology of neuronal synapses. Moreover, altered regulation of the neuronal actin cytoskeleton has been implicated in neuropsychiatric diseases such as autism spectrum disorder (ASD). Myosin XVI is a neuronally expressed unconventional myosin known to bind the WAVE regulatory complex (WRC), a regulator of filamentous actin (F-actin) polymerization. Notably, the gene encoding the myosin's heavy chain ( ) shows genetic association with neuropsychiatric disorders including ASD. Here, we investigated whether myosin XVI plays a role for actin cytoskeleton regulation in the dendritic spines of cerebellar Purkinje cells (PCs), a neuronal cell type crucial for motor learning, social cognition and vocalization. We provide evidence that both myosin XVI and the WRC component WAVE1 localize to PC spines. Fluorescence recovery after photobleaching (FRAP) analysis of GFP-actin in cultured PCs shows that knockout as well as PC-specific knockdown, lead to faster F-actin turnover in the dendritic spines of PCs. We also detect accelerated F-actin turnover upon interference with the WRC, and upon inhibition of Arp2/3 that drives formation of branched F-actin downstream of the WRC. In contrast, inhibition of formins that are responsible for polymerization of linear actin filaments does not cause faster F-actin turnover. Together, our data establish myosin XVI as a regulator of the postsynaptic actin cytoskeleton and suggest that it is an upstream activator of the WRC-Arp2/3 pathway in PC spines. Furthermore, ultra-structural and electrophysiological analyses of knockout cerebellum reveals the presence of reduced numbers of synaptic vesicles at presynaptic terminals in the absence of the myosin. Therefore, we here define myosin XVI as an F-actin regulator important for presynaptic organization in the cerebellum.
Editor: Switzerland: Frontiers Research Foundation
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Myosin XVI Regulates Actin Cytoskeleton Dynamics in Dendritic Spines of Purkinje Cells and Affects Presynaptic Organization

Roesler, Mona Katrin ; Lombino, Franco Luis ; Freitag, Sandra ; Schweizer, Michaela ; Hermans-Borgmeyer, Irm ; Schwarz, Jürgen R ; Kneussel, Matthias ; Wagner, Wolfgang

Switzerland: Frontiers Research Foundation

Buscando em bases de dados remotas. Favor aguardar.