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Improvement of indirect immunofluorescence technique to detect antineutrophil cytoplasmic antibodies and its impact on test positivity rate

Zarur, E B ; Rodrigues, S H ; Ambrogini, Jr, O ; Ferraz, M L G ; Mastroianni-Kirsztajn, G ; Andrade, L E C ; Souza, A W S de

Brazilian journal of medical and biological research, 2023-01, Vol.56 (1), p.e12636-e12636 [Periódico revisado por pares]

Brazil: Associacao Brasileira de Divulgacao Cientifica (ABDC)

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Título:
Improvement of indirect immunofluorescence technique to detect antineutrophil cytoplasmic antibodies and its impact on test positivity rate
Autor: Zarur, E B ; Rodrigues, S H ; Ambrogini, Jr, O ; Ferraz, M L G ; Mastroianni-Kirsztajn, G ; Andrade, L E C ; Souza, A W S de
Assuntos: ANCA-associated vasculitis ; Antibodies, Antineutrophil Cytoplasmic ; Antineutrophil cytoplasmic antibodies ; Autoantibodies ; Autoimmune hepatitis ; BIOLOGY ; Chronic active hepatitis ; Cross-Sectional Studies ; Diagnosis ; Enzyme-Linked Immunosorbent Assay ; Fluorescent antibody technique ; Fluorescent Antibody Technique, Indirect ; Health aspects ; Humans ; Immunofluorescence ; Measurement ; MEDICINE, RESEARCH & EXPERIMENTAL ; Methods ; MPO-ANCA ; Myeloblastin ; Peroxidase ; PR3-ANCA ; Ulcerative colitis ; Vasculitis
É parte de: Brazilian journal of medical and biological research, 2023-01, Vol.56 (1), p.e12636-e12636
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: The indirect immunofluorescence (IIF) technique for antineutrophil cytoplasmic antibodies (ANCA) detection is subject to substantial differences across laboratories. This study aimed to assess the impact of improvements in the IIF-ANCA technique on the positivity rate of ANCA tests. A cross-sectional study was performed with serum samples from patients with ANCA-associated vasculitis (AAV), autoimmune hepatitis (AIH), and ulcerative colitis (UC). A paired analysis was performed for IIF-ANCA results using the traditional method and a modified protocol after a series of specific adjustments in the technique based on the protocol of IIF-ANCA test performed at a nation-wide private laboratory in Brazil. ANCA specificity was assessed by ELISA for anti-proteinase 3 (PR3) and anti-myeloperoxidase (MPO) antibodies. Sixty-one patients were evaluated. The positivity rate of IIF-ANCA tests at disease presentation performed at the University reference laboratory was 32.3% in AAV, AIH, and UC patients, whereas the positivity rates of IIF-ANCA and ELISA tests in other laboratories were 75.0 and 72.7%, respectively. After modifications in the IIF-ANCA technique, there was a significant increase in the positivity rate (14.8 vs 34.3%; P=0.0002) and in median titers [1/40 (1/30-1/160) vs 1/80 (1/40-1/80); P=0.0003] in AAV, AIH, and UC patients. UC had the highest increment in positive results from 5.3 to 36.8%. There was poor agreement between MPO- or PR3-ANCA and both IIF-ANCA techniques. In conclusion, modifications in the IIF-ANCA protocol led to a significant improvement in its positivity rate and titers.
Editor: Brazil: Associacao Brasileira de Divulgacao Cientifica (ABDC)
Idioma: Inglês;Português

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Improvement of indirect immunofluorescence technique to detect antineutrophil cytoplasmic antibodies and its impact on test positivity rate

Zarur, E B ; Rodrigues, S H ; Ambrogini, Jr, O ; Ferraz, M L G ; Mastroianni-Kirsztajn, G ; Andrade, L E C ; Souza, A W S de

Brazil: Associacao Brasileira de Divulgacao Cientifica (ABDC)

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