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Determining the optimal method for DNA isolation from fruit jams

SOVOVÁ, Tereza ; KŘÍŽOVÁ, Barbora ; OVESNÁ, Jaroslava

Czech Journal of Food Sciences, 2018-01, Vol.36 (2), p.126-132 [Periódico revisado por pares]

Prague: Czech Academy of Agricultural Sciences (CAAS)

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  • Título:
    Determining the optimal method for DNA isolation from fruit jams
  • Autor: SOVOVÁ, Tereza ; KŘÍŽOVÁ, Barbora ; OVESNÁ, Jaroslava
  • Assuntos: Additives ; Amplification ; Chloroplasts ; Deoxyribonucleic acid ; DNA ; dna amplifiability ; dna extraction ; food analysis ; food quality ; Fruits ; Nucleotide sequence ; Optical density ; Polymerase chain reaction ; Spectrophotometry ; tRNA Leu
  • É parte de: Czech Journal of Food Sciences, 2018-01, Vol.36 (2), p.126-132
  • Descrição: DNA extraction is a crucial step in PCR analysis especially when analysing food samples that can be degraded and can potentially contain PCR-inhibiting substances. In this study, we compared the suitability of three DNA extraction methods – two kits: DNeasy® Plant Mini Kit and NucleoSpin® Food, and the CTAB method – for DNA extraction from commercial fruit jams. Fourteen jams with different contents of fruit, sugar and other additives were extracted in triplicate using the above-mentioned methods directly and after a washing step. The concentration and optical density were analysed using UV spectrophotometry and the amplifiability of the obtained DNA was evaluated using a PCR assay targeting a sequence coding for chloroplast tRNA-Leu. Samples isolated using the NucleoSpin® Food kit contained non-amplifiable DNA in eight cases, and samples isolated using the CTAB method could not be quantified. The DNeasy® Plant Mini Kit thus proved to be the most suitable method, since well-amplifiable DNA was obtained for all the analysed samples.
  • Editor: Prague: Czech Academy of Agricultural Sciences (CAAS)
  • Idioma: Inglês;Tcheco;Eslovaco
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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