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1'-O-methyl-averantin isolated from the endolichenic fungus Jackrogersella sp. EL001672 suppresses colorectal cancer stemness via sonic Hedgehog and Notch signaling

Varlı, Mücahit ; Lee, Eun-Young ; Yang, Yi ; Zhou, Rui ; Taş, İsa ; Pulat, Sultan ; Gamage, Chathurika D B ; Park, So-Yeon ; Hur, Jae-Seoun ; Nam, Sang-Jip ; Kim, Hangun

Scientific reports, 2023-02, Vol.13 (1), p.2811-2811, Article 2811 [Revista revisada por pares]

England: Nature Publishing Group

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1'-O-methyl-averantin isolated from the endolichenic fungus Jackrogersella sp. EL001672 suppresses colorectal cancer stemness via sonic Hedgehog and Notch signaling
Autor: Varlı, Mücahit ; Lee, Eun-Young ; Yang, Yi ; Zhou, Rui ; Taş, İsa ; Pulat, Sultan ; Gamage, Chathurika D B ; Park, So-Yeon ; Hur, Jae-Seoun ; Nam, Sang-Jip ; Kim, Hangun
Materias: Acetone ; Acetone - metabolism ; Antitumor activity ; Bmi protein ; Cancer ; CD44 antigen ; Cell culture ; Cell differentiation ; Cell Line, Tumor ; Cell self-renewal ; Cell viability ; Cetraria ; Colorectal cancer ; Colorectal carcinoma ; Colorectal Neoplasms - pathology ; Column chromatography ; Cytotoxicity ; Fungi ; Hedgehog protein ; Hedgehog Proteins - metabolism ; Humans ; Lichens ; Lichens - metabolism ; Neoplastic Stem Cells - metabolism ; Secondary metabolites ; Stem cells ; Tumor cell lines ; Western blotting ; Xylariales - metabolism
Es parte de: Scientific reports, 2023-02, Vol.13 (1), p.2811-2811, Article 2811
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descripción: Endolichenic fungi are host organisms that live on lichens and produce a wide variety of secondary metabolites. Colorectal cancer stem cells are capable of self-renewal and differentiation into cancer cells, which makes cancers difficult to eradicate. New alternative therapeutics are needed to inhibit the growth of tumor stem cells. This study examined the ability of an extract of Jackrogersella sp. EL001672 (derived from the lichen Cetraria sp.) and the isolated compound 1'-O-methyl-averantin to inhibit development of cancer stemness. The endolichenic fungus Jackrogersella sp. EL001672 (KACC 83021BP), derived from Cetraria sp., was grown in culture medium. The culture broth was extracted with acetone to obtain a crude extract. Column chromatography and reverse-phase HPLC were used to isolate an active compound. The anticancer activity of the extract and the isolated compound was evaluated by qRT-PCR and western blotting, and in cell viability, spheroid formation, and reporter assays. The acetone extract of EL001672 did not affect cell viability. However, 1'-O-methyl-averantin showed cytotoxic effects against cancer cell lines at 50 μg/mL and 25 μg/mL. Both the crude extract and 1'-O-methyl-averantin suppressed spheroid formation in CRC cell lines, and downregulated expression of stemness markers ALDH1, CD44, CD133, Lgr-5, Msi-1, and EphB1. To further characterize the mechanism underlying anti-stemness activity, we examined sonic Hedgehog and Notch signaling. The results showed that the crude extract and the 1'-O-methyl-averantin inhibited Gli1, Gli2, SMO, Bmi-1, Notch-1, Hes-1, and the CSL complex. Consequently, an acetone extract and 1'-O-methyl-averantin isolated from EL001672 suppresses colorectal cancer stemness by regulating the sonic Hedgehog and Notch signaling pathways.
Editor: England: Nature Publishing Group
Idioma: Inglés

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1'-O-methyl-averantin isolated from the endolichenic fungus Jackrogersella sp. EL001672 suppresses colorectal cancer stemness via sonic Hedgehog and Notch signaling

Varlı, Mücahit ; Lee, Eun-Young ; Yang, Yi ; Zhou, Rui ; Taş, İsa ; Pulat, Sultan ; Gamage, Chathurika D B ; Park, So-Yeon ; Hur, Jae-Seoun ; Nam, Sang-Jip ; Kim, Hangun

England: Nature Publishing Group

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