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Surface Architecture Influences the Rigidity of Candida albicans Cells

Le, Phuc H ; Nguyen, Duy H K ; Medina, Arturo Aburto ; Linklater, Denver P ; Loebbe, Christian ; Crawford, Russell J ; MacLaughlin, Shane ; Ivanova, Elena P

Nanomaterials (Basel, Switzerland), 2022-02, Vol.12 (3), p.567 [Periódico revisado por pares]

Switzerland: MDPI AG

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  • Título:
    Surface Architecture Influences the Rigidity of Candida albicans Cells
  • Autor: Le, Phuc H ; Nguyen, Duy H K ; Medina, Arturo Aburto ; Linklater, Denver P ; Loebbe, Christian ; Crawford, Russell J ; MacLaughlin, Shane ; Ivanova, Elena P
  • Assuntos: Actin ; Atomic force microscopy ; biofilm formation ; Biofilms ; Candida albicans ; Cell adhesion ; cell rigidity ; Contact angle ; Cytology ; Experiments ; Extracellular polymers ; Fungicides ; Kurtosis ; Mechanical properties ; Morphology ; Rigidity ; Scanning electron microscopy ; Skewness ; surface architecture ; Surface properties ; Surface roughness ; Titanium ; Topography ; Yeast ; Yeasts ; Young’s modulus
  • É parte de: Nanomaterials (Basel, Switzerland), 2022-02, Vol.12 (3), p.567
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
    These authors contributed equally to this work.
  • Descrição: Atomic force microscopy (AFM) was used to investigate the morphology and rigidity of the opportunistic pathogenic yeast, ATCC 10231, during its attachment to surfaces of three levels of nanoscale surface roughness. Non-polished titanium (npTi), polished titanium (pTi), and glass with respective average surface roughness ( ) values of 389 nm, 14 nm, and 2 nm, kurtosis ( ) values of 4, 16, and 4, and skewness ( ) values of 1, 4, and 1 were used as representative examples of each type of nanoarchitecture. Thus, npTi and glass surfaces exhibited similar and values but highly disparate . cells that had attached to the pTi surfaces exhibited a twofold increase in rigidity of 364 kPa compared to those yeast cells attached to the surfaces of npTi (164 kPa) and glass (185 kPa). The increased rigidity of the cells on pTi was accompanied by a distinct round morphology, condensed F-actin distribution, lack of cortical actin patches, and the negligible production of cell-associated polymeric substances; however, an elevated production of loose extracellular polymeric substances (EPS) was observed. The differences in the physical response of cells attached to the three surfaces suggested that the surface nanoarchitecture (characterized by skewness and kurtosis), rather than average surface roughness, could directly influence the rigidity of the cells. This work contributes to the next-generation design of antifungal surfaces by exploiting to control the extent of biofilm formation undertaken by yeast pathogens and highlights the importance of performing a detailed surface roughness characterization in order to identify and discriminate between the surface characteristics that may influence the extent of cell attachment and the subsequent behavior of the attached cells.
  • Editor: Switzerland: MDPI AG
  • Idioma: Inglês
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