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Revisiting double diffusion encoding MRS in the mouse brain at 11.7T: Which microstructural features are we sensitive to?

Vincent, Mélissa ; Palombo, Marco ; Valette, Julien

NeuroImage (Orlando, Fla.), 2020-02, Vol.207, p.116399-116399, Article 116399 [Periódico revisado por pares]

United States: Elsevier Inc

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Título:
Revisiting double diffusion encoding MRS in the mouse brain at 11.7T: Which microstructural features are we sensitive to?
Autor: Vincent, Mélissa ; Palombo, Marco ; Valette, Julien
Assuntos: Analysis ; Animals ; Anisotropy ; Bioengineering ; Brain ; Brain - pathology ; Brain - physiology ; Cell body ; Cell culture ; Computer science ; Cytology ; Diffusion ; Diffusion Magnetic Resonance Imaging - methods ; Image Processing, Computer-Assisted - methods ; Imaging ; Inositol ; Life Sciences ; Magnetic resonance imaging ; Magnetic resonance spectroscopy ; Magnetic Resonance Spectroscopy - methods ; Medical imaging equipment ; Metabolites ; Mice, Inbred C57BL ; Morphology ; N-Acetylaspartate ; Nervous system diseases ; Neurons - pathology ; Neurons - physiology ; Neurons and Cognition ; NMR ; Nuclear magnetic resonance ; Nuclear magnetic resonance spectroscopy ; Spectrum analysis ; White Matter - pathology ; White Matter - physiology
É parte de: NeuroImage (Orlando, Fla.), 2020-02, Vol.207, p.116399-116399, Article 116399
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SourceType-Scholarly Journals-1
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Descrição: Brain metabolites, such as N-acetylaspartate or myo-inositol, are constantly probing their local cellular environment under the effect of diffusion. Diffusion-weighted NMR spectroscopy therefore presents unparalleled potential to yield cell-type specific microstructural information. Double diffusion encoding (DDE) consists in applying two diffusion blocks, where gradient’s direction in the second block is varied during the course of the experiment. Unlike single diffusion encoding, DDE measurements at long mixing time display some angular modulation of the signal amplitude which reflects microscopic anisotropy (μA), while requiring relatively low gradient strength. This angular dependence has been formerly used to quantify cell fiber diameter using a model of isotropically oriented infinite cylinders. However, how additional features of the cell microstructure (such as cell body diameter, fiber length and branching) may also influence the DDE signal has been little explored. Here, we used a cryoprobe as well as state-of-the-art post-processing to perform DDE acquisitions with high accuracy and precision in the mouse brain at 11.7 T. We then compared our results to simulated DDE datasets obtained in various 3D cell models in order to pinpoint which features of cell morphology may influence the most the angular dependence of the DDE signal. While the infinite cylinder model poorly fits our experimental data, we show that incorporating branched fiber structure in our model allows more realistic interpretation of the DDE signal. Lastly, data acquired in the short mixing time regime suggest that some sensitivity to cell body diameter might be retrieved, although additional experiments would be required to further support this statement.
Editor: United States: Elsevier Inc
Idioma: Inglês

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Revisiting double diffusion encoding MRS in the mouse brain at 11.7T: Which microstructural features are we sensitive to?

Vincent, Mélissa ; Palombo, Marco ; Valette, Julien

United States: Elsevier Inc

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