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Participation of cytokines in the necrotic-inflammatory lesions in the heart and skeletal muscles of Calomys callosus infected with Trypanosoma cruzi

Magalhães-Santos, Isis F ; Andrade, Sonia G

Memórias do Instituto Oswaldo Cruz, 2005-08, Vol.100 (5), p.555-561 [Peer Reviewed Journal]

Brazil: Fundação Oswaldo Cruz, Fiocruz

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Title:
Participation of cytokines in the necrotic-inflammatory lesions in the heart and skeletal muscles of Calomys callosus infected with Trypanosoma cruzi
Author: Magalhães-Santos, Isis F ; Andrade, Sonia G
Subjects: Animals ; Calomys callosus ; Chagas Disease - metabolism ; Chagas Disease - pathology ; cytokines ; Cytokines - metabolism ; Fibrosis - parasitology ; Fibrosis - pathology ; gamma interferon ; Immunohistochemistry ; Interferon-gamma - metabolism ; Male ; Muscle, Skeletal - parasitology ; Muscle, Skeletal - pathology ; Myocardium - pathology ; PARASITOLOGY ; Rodentia ; Transforming Growth Factor beta - metabolism ; TROPICAL MEDICINE ; Trypanosoma cruzi ; tumor growth factor-beta ; tumor necrosis factor-alpha ; Tumor Necrosis Factor-alpha - metabolism
Is Part Of: Memórias do Instituto Oswaldo Cruz, 2005-08, Vol.100 (5), p.555-561
Notes: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Description: Calomys callosus , a sylvatic reservoir of Trypanosoma cruzi , when infected with the Colombian strain (Biodeme Type III, T. cruzi I ) develops necrotic-inflammatory lesions and intense early fibrogenesis in the heart and skeletal muscles, that spontaneously regress. Participation of pro-inflammatory and pro-fibrogenic cytokines, such as tumor necrosis factor-alpha (TNF-α , gamma interferon (IFN-γ) , and tumor growth factor-beta (TGF-β), in the pathogenesis of the lesions is herein studied. Eighty C. callosus weighing 20 to 30 g were used. Seventy of them were inoculated with the Colombian strain (105 blood forms) and 10 were maintained as intact non-infected controls. After infection, C. callosus were sacrificed at different time-points from 15 to 70 days. The heart and skeletal muscle were processed for histopathology and cryopreserved for immunohistochemistry. Early necrotic lesions of parasitized skeletal muscle and myocardium with intense inflammatory lesions were present. Search for the in situ presence of TNF-α and IFN-γ, was performed using rat-IgG anti-mouse antibodies against these cytokines. For the in situ search of TGF-β, rabbit IgG anti-mouse antibodies were used. Immunolabeling of the cytokines in tissues of infected C. callosus was successful. The cytokines TNF-α, IFN-γ, and TGF-β were detected in the cytoplasm of macrophages and in the necrotic material from 15 to 45 days post-infection, decreasing their intensity until complete disappearance by the 65th day, which correlated with subsiding histopathological lesions. These findings suggest the participation of these cytokines in the control of parasite multiplication, in the development of an early fibrogenesis and in the regression of fibrotic-inflammatory lesions observed in C. callosus.
Publisher: Brazil: Fundação Oswaldo Cruz, Fiocruz
Language: English;Portuguese

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Participation of cytokines in the necrotic-inflammatory lesions in the heart and skeletal muscles of Calomys callosus infected with Trypanosoma cruzi

Magalhães-Santos, Isis F ; Andrade, Sonia G

Brazil: Fundação Oswaldo Cruz, Fiocruz

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