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A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model

Souza, T.F.G ; Pierdona, T.M ; Macedo, F.S ; Aquino, P.E.A ; Rangel, G.F.P ; Duarte, R.S ; Silva, L.M.A ; Viana, G.S.B ; Alves, A.P.N.N ; Montenegro, R.C ; Wilke, D.V ; Silveira, E.R ; Alencar, N.M.N

Brazilian journal of medical and biological research, 2021-01, Vol.54 (9), p.1-e10700 [Periódico revisado por pares]

Ribeirao Preto: Associacao Brasileira de Divulgacao Cientifica (ABDC)

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Título:
A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model
Autor: Souza, T.F.G ; Pierdona, T.M ; Macedo, F.S ; Aquino, P.E.A ; Rangel, G.F.P ; Duarte, R.S ; Silva, L.M.A ; Viana, G.S.B ; Alves, A.P.N.N ; Montenegro, R.C ; Wilke, D.V ; Silveira, E.R ; Alencar, N.M.N
Assuntos: Analysis ; BIOLOGY ; Burn wounds ; Burns and scalds ; Cell migration ; Cell proliferation ; Collagen ; Contraction ; Human keratinocytes ; Inflammation ; Instrument industry ; Interleukin 10 ; International economic relations ; MEDICINE, RESEARCH & EXPERIMENTAL ; Methanol ; Methanol fraction ; Peroxidase ; Proline ; Sideroxylon ; Sideroxylon obtusifolium (Roem. & Schult.) ; Silver sulfadiazine ; Sulfadiazine ; Vascular endothelial growth factor ; Wound healing
É parte de: Brazilian journal of medical and biological research, 2021-01, Vol.54 (9), p.1-e10700
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
Descrição: It was previously demonstrated that the methanol fraction of Sideroxylon obtusifolium (MFSOL) promoted anti-inflammatory and healing activity in excisional wounds. Thus, the present work investigated the healing effects of MFSOL on human keratinocyte cells (HaCaT) and experimental burn model injuries. HaCaT cells were used to study MFSOL's effect on cell migration and proliferation rates. Female Swiss mice were subjected to a second-degree superficial burn protocol and divided into four treatment groups: Vehicle, 1.0% silver sulfadiazine, and 0.5 or 1.0% MFSOL Cream (CrMFSOL). Samples were collected to quantify the inflammatory mediators, and histological analyses were performed after 3, 7, and 14 days. The results showed that MFSOL (50 [micro]g/mL) stimulated HaCaT cells by increasing proliferation and migration rates. Moreover, 0.5% CrMFSOL attenuated myeloperoxidase (MPO) activity and also stimulated the release of interleukin (IL)-1[beta] and IL-10 after 3 days of treatment. CrMFSOL (0.5%) also enhanced wound contraction, promoted improvement of tissue remodeling, and increased collagen production after 7 days and VEGF release after 14 days. Therefore, MFSOL stimulated human keratinocyte (HaCaT) cells and improved wound healing via modulation of inflammatory mediators of burn injuries. Keywords: Sideroxylon obtusifolium (Roem. & Schult.); Methanol fraction; Human keratinocytes; Wound healing; Burn wounds
Editor: Ribeirao Preto: Associacao Brasileira de Divulgacao Cientifica (ABDC)
Idioma: Inglês;Português

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A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model

Souza, T.F.G ; Pierdona, T.M ; Macedo, F.S ; Aquino, P.E.A ; Rangel, G.F.P ; Duarte, R.S ; Silva, L.M.A ; Viana, G.S.B ; Alves, A.P.N.N ; Montenegro, R.C ; Wilke, D.V ; Silveira, E.R ; Alencar, N.M.N

Ribeirao Preto: Associacao Brasileira de Divulgacao Cientifica (ABDC)

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