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Cellular Mechanosensitivity: Validation of an Adaptable 3D-Printed Device for Microindentation

Capponi, Giulio ; Zambito, Martina ; Neri, Igor ; Cottone, Francesco ; Mattarelli, Maurizio ; Vassalli, Massimo ; Caponi, Silvia ; Florio, Tullio

Nanomaterials (Basel, Switzerland), 2022-08, Vol.12 (15), p.2691 [Periódico revisado por pares]

Basel: MDPI AG

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Título:
Cellular Mechanosensitivity: Validation of an Adaptable 3D-Printed Device for Microindentation
Autor: Capponi, Giulio ; Zambito, Martina ; Neri, Igor ; Cottone, Francesco ; Mattarelli, Maurizio ; Vassalli, Massimo ; Caponi, Silvia ; Florio, Tullio
Assuntos: 3D printing ; Actuation ; Adherent cells ; Antibiotics ; Downstream effects ; Environmental effects ; Homeostasis ; Ion channels ; Laboratories ; Measuring instruments ; Mechanical stimuli ; mechanobiology ; mechanosensitivity ; Mechanotransduction ; Membrane proteins ; Microscopy ; Physiology ; piezo1 ; Three dimensional printing
É parte de: Nanomaterials (Basel, Switzerland), 2022-08, Vol.12 (15), p.2691
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Mechanotransduction refers to the cellular ability to sense mechanical stimuli from the surrounding environment and convert them into biochemical signals that regulate cellular physiology and homeostasis. Mechanosensitive ion channels (MSCs), especially ones of Piezo family (Piezo1 and Piezo2), play a crucial role in mechanotransduction. These transmembrane proteins directly react to mechanical cues by triggering the onset of an ionic current. The relevance of this mechanism in driving physiology and pathology is emerging, and there is a growing need for the identification of an affordable and reliable assay to measure it. Setting up a mechanosensitivity assay requires exerting a mechanical stimulus on single cells while observing the downstream effects of channels opening. We propose an open-hardware approach to stimulate single adherent cells through controlled microindentation, using a 3D-printed actuation platform. We validated the device by measuring the mechanosensitivity of a neural mice cell line where the expression level and activity of Piezo1 were genetically and pharmacologically manipulated. Moreover, this extremely versatile device could be integrated with different read-out technologies, offering a new tool to improve the understanding of mechanotransduction in living cells.
Editor: Basel: MDPI AG
Idioma: Inglês

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Cellular Mechanosensitivity: Validation of an Adaptable 3D-Printed Device for Microindentation

Capponi, Giulio ; Zambito, Martina ; Neri, Igor ; Cottone, Francesco ; Mattarelli, Maurizio ; Vassalli, Massimo ; Caponi, Silvia ; Florio, Tullio

Basel: MDPI AG

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