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Anti-SARS-CoV-2 Activity of Ampelozizyphus amazonicus (Saracura-Mirá): Focus on the Modulation of the Spike-ACE2 Interaction by Chemically Characterized Bark Extracts by LC-DAD-APCI-MS/MS

Campos, Mariana Freire ; Mendonça, Simony Carvalho ; Peñaloza, Evelyn Maribel Condori ; de Oliveira, Beatriz A C ; Rosa, Alice S ; Leitão, Gilda Guimarães ; Tucci, Amanda R ; Ferreira, Vivian Neuza S ; Oliveira, Thamara Kelcya F ; Miranda, Milene Dias ; Allonso, Diego ; Leitão, Suzana Guimarães

Molecules (Basel, Switzerland), 2023-04, Vol.28 (7), p.3159 [Periódico revisado por pares]

Switzerland: MDPI AG

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Anti-SARS-CoV-2 Activity of Ampelozizyphus amazonicus (Saracura-Mirá): Focus on the Modulation of the Spike-ACE2 Interaction by Chemically Characterized Bark Extracts by LC-DAD-APCI-MS/MS
Autor: Campos, Mariana Freire ; Mendonça, Simony Carvalho ; Peñaloza, Evelyn Maribel Condori ; de Oliveira, Beatriz A C ; Rosa, Alice S ; Leitão, Gilda Guimarães ; Tucci, Amanda R ; Ferreira, Vivian Neuza S ; Oliveira, Thamara Kelcya F ; Miranda, Milene Dias ; Allonso, Diego ; Leitão, Suzana Guimarães
Assuntos: ACE2 ; Angiotensin-Converting Enzyme 2 ; Antiviral agents ; Antiviral Agents - pharmacology ; Bark ; chalcone glycosides ; Coronaviruses ; COVID-19 ; Drug development ; Ethanol ; flavonoids ; Natural products ; Pandemics ; Peptides ; Plant Bark ; Plant extracts ; Protein Binding ; Proteins ; Replication ; Rhamnaceae ; Saponins ; SARS-CoV-2 ; Severe acute respiratory syndrome coronavirus 2 ; spike protein ; Tandem Mass Spectrometry ; Viral diseases
É parte de: Molecules (Basel, Switzerland), 2023-04, Vol.28 (7), p.3159
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Traditional medicine shows several treatment protocols for COVID-19 based on natural products, revealing its potential as a possible source of anti-SARS-CoV-2 agents. is popularly used in the Brazilian Amazon as a fortifier and tonic, and recently, it has been reported to relieve COVID-19 symptoms. This work aimed to investigate the antiviral potential of , focusing on the inhibition of spike and ACE2 receptor interaction, a key step in successful infection. Although saponins are the major compounds of this plant and often reported as its active principles, a polyphenol-rich extract was the best inhibitor of the spike and ACE2 interaction. Chemical characterization of bark extracts by LC-DAD-APCI-MS/MS before and after clean-up steps for polyphenol removal showed that the latter play an essential role in maintaining this activity. The effects of the extracts on viral replication were also assessed, and all samples (aqueous and ethanol extracts) demonstrated in vitro activity, inhibiting viral titers in the supernatant of Calu-3 cells after 24 hpi. By acting both in the SARS-CoV-2 cell entry process and its replication, bark extracts stand out as a multitarget agent, highlighting the species as a promising candidate in the development of anti-SARS-CoV-2 drugs.
Editor: Switzerland: MDPI AG
Idioma: Inglês

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Anti-SARS-CoV-2 Activity of Ampelozizyphus amazonicus (Saracura-Mirá): Focus on the Modulation of the Spike-ACE2 Interaction by Chemically Characterized Bark Extracts by LC-DAD-APCI-MS/MS

Switzerland: MDPI AG

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