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CONCEPT AND STRATEGY FOR THE DEVELOPMENT OF A GENERAL PURPOSE SERUM-FREE MEDIUM FOR ANCHORAGE-DEPENDENT CELLS

Kaspi, L. ; Fiorentini, D. ; Talmon, R. ; Levi, R. ; Bennett, G.

Production of Biologicals from Animal Cells in Culture, 1991, p.137-139

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Título:
CONCEPT AND STRATEGY FOR THE DEVELOPMENT OF A GENERAL PURPOSE SERUM-FREE MEDIUM FOR ANCHORAGE-DEPENDENT CELLS
Autor: Kaspi, L. ; Fiorentini, D. ; Talmon, R. ; Levi, R. ; Bennett, G.
É parte de: Production of Biologicals from Animal Cells in Culture, 1991, p.137-139
Descrição: Most so called multi-purpose serum-free media (SFM) are expensive sophisticated mixtures of many hormones and growth factors, or contain inexpensive, undefined ingredients. This work is based on the concept that different anchorage-dependent cell types might share basic requirements, and thus a simple general purpose medium could be turned into specific media for individual cell types by the addition of a few defined ingredients. The SFM developed supports long-term growth of chosen anchorage-dependent epithelial and fibroblast-type cells. Keywords: Anchorage-dependent cells, Primary cells, Cell longevity, Long-term growth, Serum-free medium, Defined medium.
Editor: Elsevier Ltd
Idioma: Inglês

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