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Validating the sensitivity of inhomogeneous magnetization transfer (ihMT) MRI to myelin with fluorescence microscopy

Duhamel, G. ; Prevost, V.H. ; Cayre, M. ; Hertanu, A. ; Mchinda, S. ; Carvalho, V.N. ; Varma, G. ; Durbec, P. ; Alsop, D.C. ; Girard, O.M.

NeuroImage (Orlando, Fla.), 2019-10, Vol.199, p.289-303 [Periódico revisado por pares]

United States: Elsevier Inc

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  • Título:
    Validating the sensitivity of inhomogeneous magnetization transfer (ihMT) MRI to myelin with fluorescence microscopy
  • Autor: Duhamel, G. ; Prevost, V.H. ; Cayre, M. ; Hertanu, A. ; Mchinda, S. ; Carvalho, V.N. ; Varma, G. ; Durbec, P. ; Alsop, D.C. ; Girard, O.M.
  • Assuntos: Animals ; Bioengineering ; Data Interpretation, Statistical ; Fluorescence microscopy ; Gray Matter - diagnostic imaging ; ihMT ; Inhomogeneous magnetization transfer ; Life Sciences ; Magnetic resonance imaging ; Magnetic Resonance Imaging - methods ; Magnetic Resonance Imaging - standards ; Mice ; Mice, Inbred C57BL ; Microscopy ; Microscopy, Fluorescence - standards ; MRI ; Myelin ; Myelin imaging ; Myelin proteolipid protein ; Myelin Sheath ; Neuroimaging ; Neurons and Cognition ; plp-GFP ; Proteins ; Sensitivity and Specificity ; White Matter - diagnostic imaging
  • É parte de: NeuroImage (Orlando, Fla.), 2019-10, Vol.199, p.289-303
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
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    ObjectType-Undefined-3
  • Descrição: Inhomogeneous Magnetization Transfer (ihMT) is a development from the MT MRI technique. IhMT can be considered as a dipolar order relaxation time (T1D) weighted imaging modality whose signal has shown an enhanced selectivity for myelin-rich structures. However, a formal validation of the ihMT sensitivity relative to a gold standard myelin density measurement has not yet been reported. To address this need, we compared ihMT MRI with green fluorescence protein (GFP) microscopy, in a study performed on genetically-modified plp-GFP mice, considered as a reference technique for myelin-content assessment. Various ihMT protocols consisting of variable T1D-filtering and radiofrequency power temporal distributions, were used for comparison with fluorescence microscopy. Strong and significant linear relationships (r2 (0.87–0.96), p < 0.0001) were found between GFP and ihMT ratio signals across brain regions for all tested protocol variants. Conventional MT ratios showed weaker correlations (r2 (0.24–0.78), p ≤ 0.02) and a much larger signal fraction unrelated to myelin, hence corresponding to a much lower specificity for myelin. T1D-filtering reduced the ihMT signal fraction not attributed to myelin by almost twofold relative to zero filtering suggesting that at least half of the unrelated signal has a substantially shorter T1D than myelin. Overall, these results strongly support the sensitivity of ihMT to myelin content. Validation of ihMT as a myelin sensitive technique by comparison of ihMTR values derived from various RF irradiation preparations with GFP fluorescence intensity, measured in different brain structures. [Display omitted] •Inhomogeneous magnetization transfer (ihMT) was validated as a myelin sensitive imaging technique against fluorescence microscopy.•IhMT signal was strongly and significantly correlated with myelin-related plp-GFP (proteolipid - Green Fluorescence Protein) signal.•Short dipolar relaxation time (T1D) filtering is an efficient way to reduce non-myelin contribution in ihMT signal.•MT signal was more weakly correlated with plp-GFP signal and had a much larger non-myelin contribution.•IhMT contrast can be modulated with pulse timing, leading to signals with variable sensitivity and specificity for myelin.
  • Editor: United States: Elsevier Inc
  • Idioma: Inglês
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