Idioma:

OCT-1 is over-expressed in intestinal metaplasia and intestinal gastric carcinomas and binds to, but does not transactivate, CDX2 in gastric cells

Almeida, Raquel ; Almeida, José ; Shoshkes, Michal ; Mendes, Nuno ; Mesquita, Patrícia ; Silva, Elisabete ; Van Seuningen, Isabelle ; Reis, Celso A ; Santos-Silva, Filipe ; David, Leonor

The Journal of pathology, 2005-12, Vol.207 (4), p.396-401 [Periódico revisado por pares]

Chichester, UK: John Wiley & Sons, Ltd

Texto completo disponível

Citações Citado por

Enviar para

Título:
OCT-1 is over-expressed in intestinal metaplasia and intestinal gastric carcinomas and binds to, but does not transactivate, CDX2 in gastric cells
Autor: Almeida, Raquel ; Almeida, José ; Shoshkes, Michal ; Mendes, Nuno ; Mesquita, Patrícia ; Silva, Elisabete ; Van Seuningen, Isabelle ; Reis, Celso A ; Santos-Silva, Filipe ; David, Leonor
Assuntos: Cdx2 ; CDX2 Transcription Factor ; Chronic Disease ; Electrophoretic Mobility Shift Assay ; gastric carcinoma ; Gastric Mucosa - metabolism ; Gastritis - metabolism ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Humans ; intestinal metaplasia ; Life Sciences ; Metaplasia - genetics ; Metaplasia - metabolism ; Neoplasm Proteins - metabolism ; Oct1 ; Octamer Transcription Factor-1 - metabolism ; Precancerous Conditions - genetics ; Precancerous Conditions - metabolism ; Promoter Regions, Genetic ; promoter regulation ; Stomach - pathology ; Stomach Neoplasms - genetics ; Stomach Neoplasms - metabolism ; Trans-Activators - genetics ; Trans-Activators - metabolism ; Transfection ; Tumor Cells, Cultured
É parte de: The Journal of pathology, 2005-12, Vol.207 (4), p.396-401
Notas: ark:/67375/WNG-SNWFW324-Q
FEDER, EU
Fundação Calouste Gulbenkian, Portugal - No. FC-54918
Fundação Luso-Americana para o Desenvolvimento, Portugal - No. 173/202
istex:F5F662A53A8731BB4E8BE7FDAAF9DECFE3C3CC37
Programa Operacional Ciência, Portugal
ArticleID:PATH1861
Fundação para a Ciência e Tecnologia, Portugal
Tecnologia e Inovação do Quadro Comunitário de Apoio III, Portugal - No. POCTI/CBO/39075/2001
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Intestinal metaplasia (IM) is a preneoplastic lesion of the stomach in which there is transdifferentiation of the gastric mucosa to an intestinal phenotype. The caudal‐related homeobox gene CDX2 encodes an intestine‐specific transcription factor crucial for the regulation of proliferation and differentiation of intestinal cells. In addition, CDX2 is involved in the induction of IM in the stomach. The aim of this study was to access the putative involvement of OCT‐1 in the induction of CDX2 expression de novo in gastric mucosa leading to the onset of IM. OCT‐1 protein expression was evaluated by immunohistochemistry in 31 biopsies with chronic gastritis, 15 biopsies with foci of IM and adjacent gastric mucosa and 42 gastric carcinomas. Furthermore, we evaluated OCT‐1 binding by electrophoretic mobility shift assay and activation of the CDX2 promoter by co‐transfecting a CDX2 promoter/reporter construct with an OCT‐1 expression vector in two gastric carcinoma cell lines, GP220 and MKN45. Our results show that OCT‐1 is expressed in chronic gastritis, particularly when it is adjacent to IM and is expressed in 87% of IM foci. Furthermore, 74% of the gastric carcinomas were positive for OCT‐1 and a strong association was observed between OCT‐1 expression and intestinal‐type carcinoma. We identified that OCT‐1 binds to the CDX2 promoter, although we could not see a transactivation effect in gastric carcinoma cell lines. In conclusion, we observed increased OCT‐1 expression in IM and in intestinal gastric carcinomas and identified the capacity of OCT‐1 to bind to the CDX2 promoter, although we could not demonstrate a direct effect of OCT‐1 in the transactivation of CDX2. Copyright © 2005 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Editor: Chichester, UK: John Wiley & Sons, Ltd
Idioma: Inglês

Links

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

OCT-1 is over-expressed in intestinal metaplasia and intestinal gastric carcinomas and binds to, but does not transactivate, CDX2 in gastric cells

Almeida, Raquel ; Almeida, José ; Shoshkes, Michal ; Mendes, Nuno ; Mesquita, Patrícia ; Silva, Elisabete ; Van Seuningen, Isabelle ; Reis, Celso A ; Santos-Silva, Filipe ; David, Leonor

Chichester, UK: John Wiley & Sons, Ltd

Buscando em bases de dados remotas. Favor aguardar.