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UNTERSUCHUNGEN ZUR PHYSIOLOGIE DER CARNIVOREN-DRÜSEN: IV. Mitteilung: DIE KINETIK DER CHLORIDSEKRETION DURCH DAS DRÜSENGEWEBE VON NEPENTHES

Lüttge, Ulrich

Planta, 1966-01, Vol.68 (1), p.44-56 [Periódico revisado por pares]

Springer-Verlag

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  • Título:
    UNTERSUCHUNGEN ZUR PHYSIOLOGIE DER CARNIVOREN-DRÜSEN: IV. Mitteilung: DIE KINETIK DER CHLORIDSEKRETION DURCH DAS DRÜSENGEWEBE VON NEPENTHES
  • Autor: Lüttge, Ulrich
  • É parte de: Planta, 1966-01, Vol.68 (1), p.44-56
  • Descrição: The transport of chloride in isolated tissue from Nepenthes pitchers was investigated using 36Cl-, an Aminco-Cotlove chloride-titrator for the determinations of Cl- concentrations, and KCN and $\mathrm{A}\mathrm{s}{\mathrm{O}}_{4}^{--}$ as metabolic inhibitors. The tissue was brought in contact with different experimental solutions (= medium). The surface corresponding to the outside of the pitchers was cut with a razor blade to remove the cutinized epidermal layer. At this surface the Cl- uptake from the medium is a metabolic process which depends on the Cl--concentration of the medium in a manner that corresponds to the Michaelis-Menten kinetics. The Michaelis-constant of this transport step was 3 × 10-2 M. The Cl--efflux into the medium, however, is a passive process. The opposite surface of the tissue slices (corresponding to the inside of the pitchers) carries the glands. The chloride secretion taking place here is also dependent on metabolism. In vitro it occurs even when a high gradient of chloride concentration has been set up between the medium and the solution which is in contact with the glands. In vivo the Cl--concentration of the pitcher fluid and the amount of Cl- per gram of tissue water are almost equal. The rôle of chloride in the physiology of Nepenthes is still under investigation, A correlation between the chloride content of the pitcher fluid and its enzymatic activity (Casein-test), however, could already be demonstrated.
  • Editor: Springer-Verlag
  • Idioma: Alemão

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