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Interlaboratory comparison of 25-hydroxyvitamin D assays: Vitamin D Standardization Program (VDSP) Intercomparison Study 2 - Part 1 liquid chromatography - tandem mass spectrometry (LC-MS/MS) assays - impact of 3-epi-25-hydroxyvitamin D(3) on assay performance

Wise, Stephen A. ; Camara, Johanna E. ; Burdette, Carolyn Q. ; Hahm, Grace ; Nalin, Federica ; Kuszak, Adam J. ; Merkel, Joyce ; Durazo-Arvizu, Ramón A. ; Williams, Emma L. ; Hoofnagle, Andrew N. ; Ivison, Fiona ; Fischer, Ralf ; van den Ouweland, Jody M. W. ; Ho, Chung S. ; Law, Emmett W. K. ; Simard, Jean-Nicolas ; Gonthier, Renaud ; Holmquist, Brett ; Meadows, Sarah ; Cox, Lorna ; Robyak, Kimberly ; Creer, Michael H. ; Fitzgerald, Robert ; Clarke, Michael W. ; Breen, Norma ; LUKAS, Pierre ; Cavalier, Etienne ; Sempos, Christopher T.

Springer 2022

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Título:
Interlaboratory comparison of 25-hydroxyvitamin D assays: Vitamin D Standardization Program (VDSP) Intercomparison Study 2 - Part 1 liquid chromatography - tandem mass spectrometry (LC-MS/MS) assays - impact of 3-epi-25-hydroxyvitamin D(3) on assay performance
Autor: Wise, Stephen A. ; Camara, Johanna E. ; Burdette, Carolyn Q. ; Hahm, Grace ; Nalin, Federica ; Kuszak, Adam J. ; Merkel, Joyce ; Durazo-Arvizu, Ramón A. ; Williams, Emma L. ; Hoofnagle, Andrew N. ; Ivison, Fiona ; Fischer, Ralf ; van den Ouweland, Jody M. W. ; Ho, Chung S. ; Law, Emmett W. K. ; Simard, Jean-Nicolas ; Gonthier, Renaud ; Holmquist, Brett ; Meadows, Sarah ; Cox, Lorna ; Robyak, Kimberly ; Creer, Michael H. ; Fitzgerald, Robert ; Clarke, Michael W. ; Breen, Norma ; LUKAS, Pierre ; Cavalier, Etienne ; Sempos, Christopher T.
Assuntos: 24R,25-Dihydroxyvitamin D3 (24R,25(OH)3D3) ; 25-Hydroxyvitamin D2 (25(OH)D2) ; 25-Hydroxyvitamin D3 (25(OH)D3) ; 3-epi-25-Hydroxyvitamin D3 (3-epi-25(OH)D3) ; Human health sciences ; Laboratory medicine & medical technology ; Liquid chromatography – tandem mass spectrometry (LC-MS/MS) ; Médecine de laboratoire & technologie médicale ; Sciences de la santé humaine ; Total serum 25-hydroxyvitamin D (25(OH)D)
Notas: scopus-id:2-s2.0-85106907269
Descrição: An interlaboratory comparison study was conducted by the Vitamin D Standardization Program (VDSP) to assess the performance of liquid chromatography - tandem mass spectrometry (LC-MS/MS) assays used for the determination of serum total 25-hydroxyvitamin D (25(OH)D), which is the sum of 25-hydroxyvitamin D(2) (25(OH)D(2)) and 25-hydroxyvitamin D(3) (25(OH)D(3)). A set of 50 single-donor samples was assigned target values for concentrations of 25(OH)D(2), 25(OH)D(3), 3-epi-25-hydroxyvitamin D(3) (3-epi-25(OH)D(3)), and 24R,25-dihydroxyvitamin D(3) (24R,25(OH)(2)D(3)) using isotope dilution liquid chromatography - tandem mass spectrometry (ID LC-MS/MS). VDSP Intercomparison Study 2 Part 1 includes results from 14 laboratories using 14 custom LC-MS/MS assays. Assay performance was evaluated using mean % bias compared to the assigned target values and using linear regression analysis of the test assay mean results and the target values. Only 53% of the LC-MS/MS assays met the VDSP criterion of mean % bias ≤ |±5%|. For the LC-MS/MS assays not meeting the ≤ |±5%| criterion, four assays had mean % bias of between 12 and 21%. Based on multivariable regression analysis using the concentrations of the four individual vitamin D metabolites in the 50 single-donor samples, the performance of several LC-MS/MS assays was found to be influenced by the presence of 3-epi-25(OH)D(3). The results of this interlaboratory study represent the most comprehensive comparison of LC-MS/MS assay performance for serum total 25(OH)D and document the significant impact of the lack of separation of 3-epi-25(OH)D(3) and 25(OH)D(3) on assay performance, particularly with regard to mean % bias.
Editor: Springer
Data de criação/publicação: 2022
Idioma: Inglês

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Interlaboratory comparison of 25-hydroxyvitamin D assays: Vitamin D Standardization Program (VDSP) Intercomparison Study 2 - Part 1 liquid chromatography - tandem mass spectrometry (LC-MS/MS) assays - impact of 3-epi-25-hydroxyvitamin D(3) on assay performance

Springer 2022

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