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Bioluminescence and Radiation Response of Photobacterium fischeri H-2

Lerch, I. A.

Radiat. Res. 43: 161-72(Jul 1970), 1970-07, Vol.43 (1), p.161-172, Article 161 [Periódico revisado por pares]

United States: Academic Press, Inc

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Título:
Bioluminescence and Radiation Response of Photobacterium fischeri H-2
Autor: Lerch, I. A.
Assuntos: BACTERIA ; Bioluminescence ; Clone Cells - radiation effects ; DECAY ; DETERMINATION ; DOSE RATES ; Dosimetry ; Electron beams ; ELECTRONS ; ELECTRONS/effects on activity of luciferase system in Photobacterium fischeri, measurement of bioluminescence decay time to determine high dose rate ; Irradiation ; Kinetics ; LUCIFERASE ; LUCIFERASE/radiation effects on activity of, in Photobacterium fischeri, measurement of bioluminescence decay time to determine high dose rate electron ; Luciferases - radiation effects ; LUMINESCENCE ; Luminescent Measurements ; Mathematics ; MEASUREMENT ; N28800 -Life Sciences-Radiation Effects on Microorganisms ; Oxygen ; Photobacterium ; Photobacterium - radiation effects ; Radiation damage ; Radiation dosage ; RADIATION EFFECTS ; Radiometry ; SCHIZOMYCOTA/Photobacterium fischeri, radiation effects on activity of luciferase system in, measurement of bioluminescence decay time to determine high dose rate electron ; TIME ; Time Factors
É parte de: Radiat. Res. 43: 161-72(Jul 1970), 1970-07, Vol.43 (1), p.161-172, Article 161
Notas: USDOE
Descrição: A strain of Photobacterium fischeri designated H-2 was employed in our laboratory for the purpose of conducting radiobiological studies with a pulsed linear accelerator electron beam. The bioluminescence of this organism was found to be an excellent determinant for the in vivo response of the luciferase enzyme system to various radiation protocols. Postirradiation bioluminescent activity was measured with a phototube connected in series with a log amplifier and an x-y plotter for the recording of glow curves as a function of time. It was found that bioluminescence decayed exponentially with time immediately after exposure to a high dose rate primary electron beam with a rate constant varying linearly as dose. Bioluminescence survival curves were constructed utilizing measurements obtained one to two hours postirradiation upon stabilization of the light output. Clonogenic survival was found to be a much more radiosensitive function than bioluminescent survival.
Editor: United States: Academic Press, Inc
Idioma: Inglês

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Bioluminescence and Radiation Response of Photobacterium fischeri H-2

Lerch, I. A.

United States: Academic Press, Inc

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