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Mesofluidic devices for DNA-programmed combinatorial chemistry

Weisinger, Rebecca M ; Marinelli, Robert J ; Wrenn, S Jarrett ; Harbury, Pehr B Romesberg, Floyd

PloS one, 2012-03, Vol.7 (3), p.e32299-e32299 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Mesofluidic devices for DNA-programmed combinatorial chemistry
Autor: Weisinger, Rebecca M ; Marinelli, Robert J ; Wrenn, S Jarrett ; Harbury, Pehr B
Romesberg, Floyd
Assuntos: Arrays ; Automation ; Biochemistry ; Biology ; Biopolymers ; Blotting, Southern ; Catalysis ; Cellulose ; Chemical synthesis ; Chemistry ; Combinatorial analysis ; Combinatorial chemistry ; Combinatorial Chemistry Techniques - instrumentation ; Combinatorial Chemistry Techniques - methods ; Deoxyribonucleic acid ; DNA ; DNA - genetics ; DNA biosynthesis ; DNA microarrays ; Format ; Gene expression ; Gene Library ; Hybridization ; Ligands ; Medicine ; Membranes ; Nucleic Acid Hybridization ; Permeability ; Proteins ; Reproducibility of Results ; Routing ; Small Molecule Libraries ; Technology application
É parte de: PloS one, 2012-03, Vol.7 (3), p.e32299-e32299
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Current address: Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, California, United States of America
Conceived and designed the experiments: RMW RJM SJW PBH. Performed the experiments: RMW RJM SJW PBH. Analyzed the data: RMW SJW PBH. Wrote the paper: RMW PBH.
Descrição: Hybrid combinatorial chemistry strategies that use DNA as an information-carrying medium are proving to be powerful tools for molecular discovery. In order to extend these efforts, we present a highly parallel format for DNA-programmed chemical library synthesis. The new format uses a standard microwell plate footprint and is compatible with commercially available automation technology. It can accommodate a wide variety of combinatorial synthetic schemes with up to 384 different building blocks per chemical step. We demonstrate that fluidic routing of DNA populations in the highly parallel format occurs with excellent specificity, and that chemistry on DNA arrayed into 384 well plates proceeds robustly, two requirements for the high-fidelity translation and efficient in vitro evolution of small molecules.
Editor: United States: Public Library of Science
Idioma: Inglês

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Mesofluidic devices for DNA-programmed combinatorial chemistry

Weisinger, Rebecca M ; Marinelli, Robert J ; Wrenn, S Jarrett ; Harbury, Pehr B Romesberg, Floyd

United States: Public Library of Science

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