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Microvesicles from Mesenchymal Stromal Cells Are Involved in HPC-Microenvironment Crosstalk in Myelodysplastic Patients

Muntión, Sandra ; Ramos, Teresa L ; Diez-Campelo, María ; Rosón, Beatriz ; Sánchez-Abarca, Luis Ignacio ; Misiewicz-Krzeminska, Irena ; Preciado, Silvia ; Sarasquete, María-Eugenia ; de Las Rivas, Javier ; González, Marcos ; Sánchez-Guijo, Fermín ; Del Cañizo, María-Consuelo Wagner, Wolfgang

PloS one, 2016-02, Vol.11 (2), p.e0146722-e0146722 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Microvesicles from Mesenchymal Stromal Cells Are Involved in HPC-Microenvironment Crosstalk in Myelodysplastic Patients
Autor: Muntión, Sandra ; Ramos, Teresa L ; Diez-Campelo, María ; Rosón, Beatriz ; Sánchez-Abarca, Luis Ignacio ; Misiewicz-Krzeminska, Irena ; Preciado, Silvia ; Sarasquete, María-Eugenia ; de Las Rivas, Javier ; González, Marcos ; Sánchez-Guijo, Fermín ; Del Cañizo, María-Consuelo
Wagner, Wolfgang
Assuntos: Adult ; Aged ; Aged, 80 and over ; Antigens, CD34 ; Apoptosis ; Biology and life sciences ; Bone marrow ; CD34 antigen ; CD63 antigen ; Cell Communication ; Cell cycle ; Cell signaling ; Cell Survival ; Cellular Microenvironment ; Communication ; Crosstalk ; Cytometry ; Development and progression ; DNA methylation ; Electron microscopy ; Epigenetics ; Exosomes ; Exosomes - metabolism ; Female ; Flow cytometry ; Fluorescence ; Fluorescence microscopy ; Gas vesicles ; Gene Expression ; Genetic aspects ; Hematopoietic stem cells ; Hematopoietic Stem Cells - immunology ; Hematopoietic Stem Cells - metabolism ; Hemopoiesis ; Humans ; Leukemia ; Male ; MDM2 protein ; Medicine and Health Sciences ; Mesenchymal Stromal Cells - metabolism ; Mesenchyme ; MicroRNAs ; MicroRNAs - metabolism ; Middle Aged ; miRNA ; Myelodysplastic syndrome ; Myelodysplastic syndromes ; Myelodysplastic Syndromes - genetics ; Myelodysplastic Syndromes - metabolism ; p53 Protein ; Patients ; Physiological aspects ; Polymerase chain reaction ; Progenitor cells ; Properties (attributes) ; Research and Analysis Methods ; Stem cells ; Stromal cells ; Transmission electron microscopy ; Ultracentrifugation
É parte de: PloS one, 2016-02, Vol.11 (2), p.e0146722-e0146722
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Conceived and designed the experiments: MCC FS-G SM. Performed the experiments: SM TLR LISA IMK SP MES. Analyzed the data: MCC SM FS-G TLR. Contributed reagents/materials/analysis tools: SM MDC BR MES TLR MG IMK JR. Wrote the paper: SM MCC FS-G TLR.
Competing Interests: The authors have declared that no competing interests exist.
Descrição: Exosomes/microvesicles (MVs) provide a mechanism of intercellular communication. Our hypothesis was that mesenchymal stromal cells (MSC) from myelodysplastic syndrome (MDS) patients could modify CD34+ cells properties by MVs. They were isolated from MSC from MDS patients and healthy donors (HD). MVs from 30 low-risk MDS patients and 27 HD were purified by ExoQuick-TC™ or ultracentrifugation and identified by transmission electron microscopy, flow cytometry (FC) and western blot for CD63. Incorporation of MVs into CD34+ cells was analyzed by FC, and confocal and fluorescence microscopy. Changes in hematopoietic progenitor cell (HPC) properties were assessed from modifications in microRNAs and gene expression in CD34+ cells as well as viability and clonogenic assays of CD34+ cells after MVs incorporation. Some microRNAs were overexpressed in MVs from patients MSC and two of them, miR-10a and miR-15a, were confirmed by RT-PCR. These microRNAs were transferred to CD34+ cells, modifying the expression of MDM2 and P53 genes, which was evaluated by RT-PCR and western blot. Finally, examining CD34+ cells properties after incorporation, higher cell viability (p = 0.025) and clonogenic capacity (p = 0.037) were observed when MVs from MDS patients were incorporated. In summary, we show that BM-MSC release MVs with a different cargo in MDS patients compared with HD. These structures are incorporated into HPC and modify their properties.
Editor: United States: Public Library of Science
Idioma: Inglês

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Microvesicles from Mesenchymal Stromal Cells Are Involved in HPC-Microenvironment Crosstalk in Myelodysplastic Patients

United States: Public Library of Science

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