Idioma:

Zebrafish Caudal Fin Angiogenesis Assay-Advanced Quantitative Assessment Including 3-Way Correlative Microscopy

Hlushchuk, Ruslan ; Brönnimann, Daniel ; Correa Shokiche, Carlos ; Schaad, Laura ; Triet, Ramona ; Jazwinska, Anna ; Tschanz, Stefan A ; Djonov, Valentin Ramchandran, Ramani

PloS one, 2016-03, Vol.11 (3), p.e0149281-e0149281 [Periódico revisado por pares]

United States: Public Library of Science

Texto completo disponível

Citações Citado por

Enviar para

Título:
Zebrafish Caudal Fin Angiogenesis Assay-Advanced Quantitative Assessment Including 3-Way Correlative Microscopy
Autor: Hlushchuk, Ruslan ; Brönnimann, Daniel ; Correa Shokiche, Carlos ; Schaad, Laura ; Triet, Ramona ; Jazwinska, Anna ; Tschanz, Stefan A ; Djonov, Valentin
Ramchandran, Ramani
Assuntos: Amputation ; Angiogenesis ; Animal Fins - blood supply ; Animal Fins - drug effects ; Animal Fins - physiology ; Animal models ; Animals ; Assaying ; Biology and Life Sciences ; Cancer ; Clinical trials ; Computer and Information Sciences ; Correlation analysis ; Cost-Benefit Analysis ; Danio rerio ; Fluorescence ; Fluorescence microscopy ; Genetic aspects ; Hypoxia ; In vivo methods and tests ; Kinases ; Laboratory animals ; Mathematical models ; Medicine and Health Sciences ; Microscopy ; Microscopy - economics ; Microscopy - methods ; Neovascularization ; Neovascularization, Physiologic - drug effects ; Phthalazines - pharmacology ; Physiological aspects ; Physiology ; Pyridines - pharmacology ; Qualitative analysis ; Quantitative analysis ; Regeneration ; Regeneration - drug effects ; Research and Analysis Methods ; Studies ; Substrates ; Tail ; Time Factors ; Vascular endothelial growth factor ; Zebra fish ; Zebrafish
É parte de: PloS one, 2016-03, Vol.11 (3), p.e0149281-e0149281
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Conceived and designed the experiments: RH DB AJ SAT VD. Performed the experiments: RH DB RT. Analyzed the data: RH DB CCS LS. Contributed reagents/materials/analysis tools: RH CCS AJ VD. Wrote the paper: RH DB AJ VD. Designed the software used in analysis: CCS. Supervised the design of the software used in analysis: RH SAT.
Competing Interests: The authors have declared that no competing interests exist.
Descrição: Researchers evaluating angiomodulating compounds as a part of scientific projects or pre-clinical studies are often confronted with limitations of applied animal models. The rough and insufficient early-stage compound assessment without reliable quantification of the vascular response counts, at least partially, to the low transition rate to clinics. To establish an advanced, rapid and cost-effective angiogenesis assay for the precise and sensitive assessment of angiomodulating compounds using zebrafish caudal fin regeneration. It should provide information regarding the angiogenic mechanisms involved and should include qualitative and quantitative data of drug effects in a non-biased and time-efficient way. Basic vascular parameters (total regenerated area, vascular projection area, contour length, vessel area density) were extracted from in vivo fluorescence microscopy images using a stereological approach. Skeletonization of the vasculature by our custom-made software Skelios provided additional parameters including "graph energy" and "distance to farthest node". The latter gave important insights into the complexity, connectivity and maturation status of the regenerating vascular network. The employment of a reference point (vascular parameters prior amputation) is unique for the model and crucial for a proper assessment. Additionally, the assay provides exceptional possibilities for correlative microscopy by combining in vivo-imaging and morphological investigation of the area of interest. The 3-way correlative microscopy links the dynamic changes in vivo with their structural substrate at the subcellular level. The improved zebrafish fin regeneration model with advanced quantitative analysis and optional 3-way correlative morphology is a promising in vivo angiogenesis assay, well-suitable for basic research and preclinical investigations.
Editor: United States: Public Library of Science
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Anterior Resultado 3 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Zebrafish Caudal Fin Angiogenesis Assay-Advanced Quantitative Assessment Including 3-Way Correlative Microscopy

Hlushchuk, Ruslan ; Brönnimann, Daniel ; Correa Shokiche, Carlos ; Schaad, Laura ; Triet, Ramona ; Jazwinska, Anna ; Tschanz, Stefan A ; Djonov, Valentin Ramchandran, Ramani

United States: Public Library of Science

Buscando em bases de dados remotas. Favor aguardar.