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1,25-Dihydroxyvitamin-D3 Induces Avian β-Defensin Gene Expression in Chickens

Zhang, Long ; Lu, Lu ; Li, Siming ; Zhang, Guolong ; Ouyang, Linghua ; Robinson, Kelsy ; Tang, Yanqiang ; Zhu, Qing ; Li, Diyan ; Hu, Yaodong ; Liu, Yiping Yao, Yong-Gang

PloS one, 2016-05, Vol.11 (5), p.e0154546-e0154546 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
1,25-Dihydroxyvitamin-D3 Induces Avian β-Defensin Gene Expression in Chickens
Autor: Zhang, Long ; Lu, Lu ; Li, Siming ; Zhang, Guolong ; Ouyang, Linghua ; Robinson, Kelsy ; Tang, Yanqiang ; Zhu, Qing ; Li, Diyan ; Hu, Yaodong ; Liu, Yiping
Yao, Yong-Gang
Assuntos: Animal production ; Animal sciences ; Animals ; Antibiotics ; Augmentation ; Bacterial infections ; beta-Defensins - genetics ; beta-Defensins - metabolism ; Biology and Life Sciences ; Breeding of animals ; Chickens ; Cycloheximide ; Diet ; Disease control ; Disease resistance ; Drug resistance ; Efficiency ; Embryos ; Epithelial cells ; Epithelial Cells - drug effects ; Epithelial Cells - metabolism ; Feeds ; Gastroenterology ; Gene expression ; Gene Expression Profiling ; Homeostasis ; Immunity ; Immunity, Innate - drug effects ; Immunology ; Inflammatory bowel disease ; Innate immunity ; Intestinal Mucosa - cytology ; Intestine ; Laboratory animals ; Leukocytes (mononuclear) ; Leukocytes, Mononuclear - drug effects ; Leukocytes, Mononuclear - metabolism ; Lipopolysaccharides ; Lipopolysaccharides - pharmacology ; Lu Lu ; Medicine and Health Sciences ; Peptides ; Peripheral blood mononuclear cells ; Poultry ; Rodents ; Small intestine ; Vitamin D - analogs & derivatives ; Vitamin D - pharmacology ; Vitamin D receptors ; Vitamin D3 ; Vitamin D3 24-Hydroxylase - metabolism ; Zoology
É parte de: PloS one, 2016-05, Vol.11 (5), p.e0154546-e0154546
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: The authors have declared that no competing interests exist.
Conceived and designed the experiments: LZ SL YL. Performed the experiments: LZ LL DL. Analyzed the data: LO. Contributed reagents/materials/analysis tools: GZ YT. Wrote the paper: LZ. Revised the manuscript: KR QZ YH.
Descrição: Host defense peptides (HDPs) play a critical role in innate immunity. Specific modulation of endogenous HDP synthesis by dietary compounds has been regarded as a novel approach to boost immunity and disease resistance in animal production. 1,25-dihydroxy vitamin D3 (1,25D3) is well known as a powerful HDP inducer in humans, but limited information about the effect of 1,25D3 on HDPs in poultry is available. Here, we sought to examine whether 1,25D3 could stimulate avian β-defensin (AvBD) expression in chickens. We used chicken embryo intestinal epithelial cells (CEIEPCs) and peripheral blood mononuclear cells (PBMCs) to study the effect of 1,25D3 on the expression of AvBDs. We observed that 1,25D3 is able to up-regulate the expression of several AvBDs in CEIEPCs and PBMCs, whereas it increased the amounts of AvBD4 mRNA in CEIEPCs only in the presence of lipopolysaccharide (LPS). On the other hand, LPS treatment not only inhibited the expression of CYP24A1 but also altered the expression pattern of VDR in CEIEPCs. Furthermore, AvBDs were not directly regulated by 1,25D3, as cycloheximide completely blocked 1,25D3-induced expression of AvBDs. Our observations suggest that 1,25D3 is capable of inducing AvBD gene expression and is a potential antibiotic alternative through augmentation of host innate immunity as well as disease control in chickens.
Editor: United States: Public Library of Science
Idioma: Inglês

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1,25-Dihydroxyvitamin-D3 Induces Avian β-Defensin Gene Expression in Chickens

Zhang, Long ; Lu, Lu ; Li, Siming ; Zhang, Guolong ; Ouyang, Linghua ; Robinson, Kelsy ; Tang, Yanqiang ; Zhu, Qing ; Li, Diyan ; Hu, Yaodong ; Liu, Yiping Yao, Yong-Gang

United States: Public Library of Science

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